摘要
本文基于电沉积原理,利用海藻酸钠,以碳酸钙作为交联剂,通过离子交联反应制备管状海藻酸钙水凝胶。研究电沉积时间、直流电源电压、海藻酸钠浓度和碳酸钙浓度4种电沉积参数对管状水凝胶直径的影响,同时建立水凝胶直径生长模型预测水凝胶直径,通过细胞活性实验研究制备的管状水凝胶对细胞毒性影响。实验结果表明,直流电源电压为4.5 V、电沉积时间50 s、海藻酸钠1%g/mL及碳酸钙0.5%g/mL时,所制备的水凝胶密度均匀且轮廓清晰;水凝胶直径生长模型与实验测量的水凝胶尺寸误差在5%以内,能够实现水凝胶直径的有效控制;细胞包埋在水凝胶培养10 h后,细胞存活率保持在75%以上,表明电沉积制备方法对细胞损害小,适用于细胞包埋,对组织工程体外生物支架构建具有重要价值。
The tubular calcium alginate hydrogels were prepared by using calcium carbonate(CaCO3)as crosslinker and sodium alginate through ionic crosslinking reaction.The effects of the electrodeposition time,direct current(DC)power supply voltage,sodium alginate concentration and CaCO3 concentration on the diameter of tubular hydrogel were investigated.Meanwhile,the hydrogel diameter growth model was established to predict the hydrogel diameter,and the prepared tubular hydrogel was used to study effects of cell cytotoxicity via cell activity test.The results show that the prepared hydrogel has a uniform density and a clear outline when the DC power supply voltage is 4.5 V,electrodeposition time is 50 s,sodium alginate concentration is 1%g/mL and CaCO3 concentration is 0.5%g/mL.The error between the hydrogel diameter growth model and the measured hydrogel size is less than 5%,which can effectively control the hydrogel diameter.After cell is embedded in the hydrogel for 10 h,the cell survival rate remains above 75%,indicating that the hydrogel material has little cytotoxicity and is suitable for cell embedding,which is of great value for the construction of biological scaffolds in vitro tissue engineering.
作者
曹辉煌
马立
解飞
Huihuang Cao;Li Ma;Fei Xie(School of Mechatronic Engineering and Automation,Shanghai University,Shanghai 200072,China)
出处
《高分子材料科学与工程》
EI
CAS
CSCD
北大核心
2020年第9期97-104,共8页
Polymer Materials Science & Engineering
基金
国家重点研发计划(2018YFB1309200)
国家自然科学基金资助项目(61573238)。
关键词
管状海藻酸钙水凝胶
电沉积
水凝胶生长模型
组织工程
tubular calcium alginate hydrogel
electrodeposition
hydrogel diameter growth model
tissue engineering
作者简介
通讯联系人:马立,主要从事微操作机器人技术研究,E-mail:malian@shu.edu.cn。
