摘要
目的研究中草药蒲公英水提物对临床常见肠杆菌科细菌体外抑菌作用。方法采用最小二倍稀释法检测蒲公英水提物对临床分离肠杆菌科细菌的最低抑菌浓度(MIC)和最低杀菌浓度(MBC);采用基质辅助激光解析串联飞行时间质谱仪(MALDI-TOF)检测蒲公英水提物对肠杆菌科细菌蛋白种类及含量的影响。结果蒲公英水提物对大肠埃希菌的MIC为0.125 g·mL^(-1),MBC为0.5 g·mL^(-1);对神户肠杆菌的MIC为0.125 g·mL^(-1),MBC为0.5 g·mL^(-1);对阴沟肠杆菌的MIC为0.06 g·mL^(-1),MBC为0.25 g·mL^(-1);对弗劳地枸橼酸杆菌的MIC为0.06 g·mL^(-1),MBC为0.5 g·mL^(-1);对伤寒沙门菌的MIC为0.125 g·mL^(-1),MBC为0.5 g·mL^(-1);对5种细菌蛋白种类及蛋白含量均有显著抑制作用。结论中草药蒲公英的水提物对临床常见肠杆菌科细菌的生长及蛋白合成有不同程度的抑制作用。
Objective To study the antibacterial effect of dandelion aqueous extract on Enterobacteriaceae in vitro.Methods The minimum inhibitory concentration(MIC) and minimum bactericidal concentration(MBC) of dandelion aqueous extract against Enterobacteriaceae were determined using the minimum double dilution method. The MALDITOF mass spectrometer was used to detect the effect of dandelion aqueous extract on the types and contents of Enterobacteriaceae bacteria protein. Results The MIC and MBC of dandelion for Escherichia coli were 0.125 g·mL^(-1) and 0.5 g·mL^(-1) respectively, 0.125 g·mL^(-1) and 0.5 g·mL^(-1) for Enterobacter kobei, 0.06 g·mL^(-1) and 0.25 g·mL^(-1) for Enterobacter cloacae, 0.06 g·mL^(-1) and 0.5 g·mL^(-1) for Citrobacter freundii, and 0.125 g·mL^(-1) and 0.5 g·mL^(-1) for Salmonella typhi. Dandelion could inhibit the types and contents of proteins of five strains of bacteria. Conclusion The aqueous extract of dandelion has different degrees of inhibition on the growth and protein synthesis of Enterobacteriaceae.
作者
王小宁
张维华
张向阳
窦建卫
王翠
WANG Xiaoning;ZHANG Weihua;ZHANG Xiangyang;DOU Jianwei;WANG Cui(Weinan Inspection and Testing Institute,Weinan Shaanxi 714000 China;Shaanxi Provincial People’s Hospital,Xi’an Shaanxi 710068 China;The Third Affiliated Hospital of Xi’an Jiaotong University,Xi’an Shaanxi 710068,China;School of Pharmacy,Xi’an Jiaotong University,Xi’an Shaanxi 710061,China)
出处
《中国药物警戒》
2022年第2期148-153,共6页
Chinese Journal of Pharmacovigilance
基金
国家自然科学基金资助项目(81873311,82074374,81573983)
陕西省中医药管理局课题(2019-ZZ-JC029)。
作者简介
王小宁,女,硕士,主管药师,药品安全性监测;通信作者:王翠,女,硕士,主管技师,中药抗微生物与肿瘤药效物质基础。E-mail:tracywangcui@163.com。
