摘要
目的:探寻益气复脉/参芪扶正注射液发挥补气作用的潜在靶基因及其生物学功能。方法:利用高通量转录组测序技术,考察气虚患者经益气复脉/参芪扶正注射液治疗后,其外周血单核细胞的差异表达基因、GO(Gene Ontotology)分析及KEGG(Kyoto encyclopedia of genes and genomes)分析,并进一步进行韦恩分析,明确2个制剂治疗后共同的差异基因及生物学功能,最后利用一组临床队列样本,通过实时定量PCR,验证并明确与补气密切相关的基因。结果:两组共有的GO条目,包括生物过程的有32条,细胞组分的有2条,分子功能的有6条,生物通路有10条。共表达差异基因有6个,包括DUSP1、DUSP2、FKBP5、F8A2、C9orf129 EGR1。临床队列样本显示,FKBP5的下调有统计学差异(P=0.001)。结论:MAPK信号通路是益气复脉和参芪扶正发挥补气作用的核心通路,FKBP5基因水平下调是它们发挥补气作用的分子标志。
Objective:To identify the potential target genes and biological functions related to tonifying Qi of Yiqi Fumai and Shenqi Fuzheng injection.Methods:In this study,we performed RNA-seq experiments and comparative transcriptomic analysis on pre-and post-peripheral blood mononuclear cell samples collected from Qi-deficiency patients treated with Yiqi FuMai or Shenqi Fuzheng.The significant differentially expressed genes(DEGs)for each treatment were determined;GO(Gene ontology)and KEGG analysis were assigned.Subsequently,Venn analysis was performed to identify genes and biological functions involved in both treatments.Finally,the DEGs in common were investigated in a cohort study by using real time qPCR.Results:There were common items involved in both treatments including 32 of biological process,2 of cellular component,6 of molecular function and 10 of signaling pathway.Their consistent DEGs include DUSP1,DUSP2,FKBP5,F8A2,C9orf129 and EGR1.Down-regulation on FKBP5 showed significant differences in the cohort study(P=0.001).Conclusion:MAPK signaling pathway is an action core in both treatments.Down-regulation of FKBP5 is suggested as a response bio-marker for their function on tonifying Qi.
作者
黄丽萍
乔博灵
颜雪珍
赵亭
纪昌春
杨峥
张晓霞
郭正萍
罗苓芝
HUANG Liping;QIAO Boling;YAN Xuezhen;ZhAO Ting;JI Changchun;YANG Zheng;ZHANG Xiaoxia;GUO Zhengping;LUO Lingzhi(Shaanxi Provincial Hospital of Chinese Medicine,Xi’an 710003,China)
出处
《陕西中医》
CAS
2022年第1期28-32,共5页
Shaanxi Journal of Traditional Chinese Medicine
基金
陕西省自然科学基金资助面上项目(2020JM-672)。
作者简介
通讯作者:乔博灵,E-mail:bolingq@nwu.edu.cn。
