摘要
目的探讨孤立性肺结节(SPN)患者血清和呼出气冷凝液(EBC)中微小RNA(miR)-21、miR-486检测的临床意义。方法采用实时定量聚合酶链反应(RT-qPCR)检测79例SPN患者和60例健康人(对照组)的血清及EBC中miR-21、miR-486水平,将79例SPN患者中术后被证实为肺癌的患者作为恶性组(54例),良性病变患者作为良性组(25例)。比较良性组、恶性组及对照组血清及EBC中miR-21、miR-486水平,并评价其在恶性SPN中的诊断价值。结果对照组、良性组及恶性组受试者血清和EBC中miR-21及miR-486比较差异均有统计学意义(P <0.05),其中恶性组受试者EBC和血清中miR-21水平均高于良性组及健康组,EBC和血清中miR-486水平均低于良性组及健康组(P <0.05)。受试者工作特征(ROC)曲线分析结果显示,血清和EBC中miR-21诊断恶性SPN的敏感性分别为为72.2%、83.3%,特异性分别为76.0%、64.0%。EBC和血清中miR-21联合检测诊断恶性SPN的敏感性为81.5%,特异性为76.0%。血清和EBC中miR-486诊断恶性SPN的敏感性为76.0%、80.0%,特异性分别为66.7%、85.2%。EBC和血清中miR-486联合检测诊断恶性SPN的敏感性为84.0%,特异性为92.6%。结论 SPN患者EBC和血清中miR-21、miR-486的检测对SPN患者良恶性评估具有较好的临床价值,有助于恶性SPN的早期诊断。
Objective To explore the clinical significance of detection of microRNA( miR)-21 and miR-486 in serum and exhaled breath condensate( EBC) of patients with solitary pulmonary nodules( SPN). Methods Quantitative reverse transcriptase polymerase chain reaction( RT-qPCR) was used to determine levels of miR-21 and miR-486 in serum and EBC of 79 patients with SPN and 60 healthy volunteers( control group). Patients who were diagnosed with lung cancer after operation among 79 patients with SPN were regarded as malignant group( 54 cases) and patients with benign lesions were regarded as benign group( 25 cases). Levels of miR-21 and miR-486 in serum and EBC among benign group,malignant group and control group were compared,and to evaluate their diagnostic value in malignant SPN. Results Levels of miR-21 and miR-486 in serum and EBC among control group,benign group and malignant group were significant different( P < 0. 05). Levels of miR-21 in EBC and serum of malignant group were higher than those of benign group and control group,and levels of miR-486 in EBC and serum of malignant group were lower than those of benign group and control group( P < 0. 05). Receiver operating characteristic( ROC) curve analysis showed that sensitivity of miR-21 in serum and EBC to diagnose malignant SPN was 72. 2% and 83. 3%,and the specificity was 76. 0% and 64. 0% respectively. Sensitivity of combined detection of miR-21 in EBC and serum to diagnose malignant SPN was 81. 5%,and specificity was76. 0%. Sensitivity of miR-486 in serum and EBC to diagnose malignant SPN was 76. 0% and 80. 0%,and the specificity was 66. 7% and 85. 2% respectively. Sensitivity of combined detection of miR-486 in EBC and serum to diagnose malignant SPN was 84. 0%,and the specificity was 92. 6%. Conclusion Detection of miR-21 and miR-486 in serum and EBC in patients with SPN has good clinical value and is helpful for the early diagnosis of malignant SPN.
作者
吴丹丹
陈金亮
姚苏梅
何海艳
朱杰
吕学东
Wu Dandan;Chen Jinliang;Yao Sumei;He Haiyan;Zhu Jie;Lv Xuedong(Department of Respiratory Medicine,the Second Affiliated Hospital of Nantong University,Nantong 226001,China)
出处
《临床内科杂志》
CAS
2021年第12期817-821,共5页
Journal of Clinical Internal Medicine
基金
江苏省南通市科技项目(MS12017004-3)。
作者简介
通讯作者:吕学东,E-mail:lxd174@126.com。
