摘要
目的探讨外周血单核细胞(PBMC)程序性死亡配体-2(PD-L2)分子在系统性红斑狼疮(SLE)中的应用。方法回顾性分析2019年1月至2020年1月萍乡市人民医院收治的30例SLE患者临床资料,将SLE疾病活动指数(SLEDAI)评分>5分的15例活动期狼疮性肾炎患者纳入活动期组,将SLEDAI评分≤5分的15例缓解期狼疮患者纳入缓解期组,并选取同期于萍乡市人民医院接受治疗的10例类风湿关节炎(RA)患者作为活动期阳性对照纳入RA组及接受体检的10例健康志愿者纳入正常对照组。所有入选者均接受PBMC的PD-L2分子检测,分析PBMC的PD-L2分子的表达水平在SLE患者的应用价值。结果不同性别、年龄、体重的SLE患者PD-L2分子表达水平比较,差异无统计学意义(P>0.05);活动期组患者PD-L2分子表达水平高于缓解期组患者与正常对照组患者,差异有统计学意义(P<0.05);活动期组患者PD-L2分子表达水平与RA组患者比较,差异无统计学意义(P>0.05);Pearson相关性分析发现,SLE患者SLEDAI评分与PD-L2分子表达水平呈正相关(r=0.946,P=0.000);受试者工作曲线结果显示,PD-L2分子表达水平预测SLE患者处于活动期的曲线下面积为0.820,具有较高的预测价值。结论PBMC的PD-L2分子表达水平与SLE患者病情活动程度密切相关,可辅助临床评估病情严重程度,为临床制定治疗方案提供有效参考。
Objective To investigate the application of peripheral blood mononuclear cell(PBMC)programmed death ligand-2(PD-L2)molecule in systemic lupus erythematosus(SLE).Methods The clinical data of 30 patients with SLE admitted to Pingxiang People′s Hospital from January 2019 to January 2020 were retrospectively analyzed.A total of 15 patients with lupus nephritis at active stage with SLE disease activity index(SLEDAI)score>5 points were included into the active group,a total of 15 patients with lupus at remission stage with SLEDAI score≤5 points were included into the remission group,a total of 10 patients with rheumatoid arthritis(RA)treated in Pingxiang People′s Hospital during the same period were selected as active control cases and included in the RA group,and 10 healthy volunteers who received physical examination were included in the normal control group.All selected candidates received PD-L2 molecular detection of PBMC,the application value of PD-L2 molecule expression level of PBMC in SLE patients was analyzed.Results There was no statistical significant difference in the expression level of PD-L2 molecule in SLE patients with different gender,age and weight(P>0.05);The expression level of PD-L2 molecule of patients in the active group was higher than that in patients of the remission group and the normal control group,and the differences were statistically significant(P<0.05);there was no statistical significant difference in the expression level of PD-L2 molecule of patients between the active group and the RA group(P>0.05);Pearson correlation analysis found that SLEDAI score was positively correlated with PD-L2 molecular expression level of SLE patients(r=0.946,P=0.000);the receiver operating curve was drawn,the results showed that the area under the curve of PD-L2 molecular expression level in the prediction of SLE patients at active stage was 0.820,which had high predictive value.Conclusion The expression level of PD-L2 molecule in PBMC is closely related to the degree of disease activity in SLE patients,which can assist clinical evaluation of the severity of the disease,and provide effective reference for the formulation of clinical treatment regimen.
作者
柳毓文
刘萍
胡娜
葛洪亮
彭雨薇
LIU Yu-wen;LIU Ping;HU Na;GE Hong-liang;PENG Yu-wei(Department of Rheumatology and Immunology,Pingxiang People′s Hospital,Jiangxi Province,Pingxiang337000,China)
出处
《中国当代医药》
CAS
2021年第34期79-82,共4页
China Modern Medicine
基金
江西省萍乡市科技计划项目(2018PY031)。
关键词
系统性红斑狼疮
狼疮性肾炎
外周血单核细胞程序性死亡配体-2
系统性红斑狼疮疾病活动指数
Systemic lupus erythematosus
Lupus nephritis
Peripheral blood mononuclear cell programmed death ligand-2
Activity index of systemic lupus erythematosus disease
