摘要
目的:明确钩藤炭疽病病原及其生物学特性,筛选有效防控药剂。方法:通过分离、纯化和回接试验,结合形态学和分子生物学对病原菌进行鉴定,并采用菌丝生长速率法测定7种杀菌剂对该病原菌的毒力。结果:引起钩藤炭疽病病原菌为博宁炭疽菌Colletotrichum boninense;适宜该菌株生长的温度为28℃,pH为6~7,适宜光照条件为24 h全光照,在蔗糖与硝酸钾条件下菌丝生长速度分别高于其他碳、氮源;7种不同杀菌剂中,75%肟菌·戊唑醇WG对博宁炭疽菌毒力较强,EC50达1.4925 mg/L,其次为250 g/L嘧菌酯SC,EC50为6.8886 mg/L。结论:钩藤炭疽病致病菌为博宁炭疽菌Colletotrichum boninense。
Objective: To definite the pathogen and biological characteristics of anthracnose in Uncaria hirsuta,to screen effective control fungicides.Methods: The pathogen was identified by isolation, purification, grafting test, combined with morphology and molecular biology.Mycelia growth rate method was used to determine the virulence of seven fungicides against the pathogen.Results: Colletotrichum boninense was the pathogen of anthracnose in Uncaria hirsuta.The suitable temperature for growth of the strain was 28 ℃,pH was 6 to 7 and the suitable light condition was 24 h full light.Under the conditions of sucrose and potassium nitrate, the growth rate of mycelia was higher than that of other carbon and nitrogen sources.Among the seven fungicides, 75% trifloxystrobin-tebuconazole WG had a strong toxicity to Colletotrichum boninense,with the EC50reaching 1.4925 mg/L,followed by 250 g/L azoxystrobin SC,its EC50 was 6.8886 mg/L.Conclusion: The pathogenic bacterium of anthracnose in Uncaria hirsuta was Colletotrichum boninense.
作者
邓洁
莫飞旭
石金巧
龙友华
吴小毛
张柱亭
冉飞
DENG Jie;MO Fei-xu;SHI Jin-qiao;LONG You-hua;WU Xiao-mao;ZHANG Zhu-ting;RAN Fei(Institute of Crop Protection,Guizhou University,Guiyang 550025,China;Kaili College,Kaili 556011,China)
出处
《中药材》
CAS
北大核心
2020年第6期1303-1307,共5页
Journal of Chinese Medicinal Materials
基金
黔东南州科技计划项目[黔东南科合J字(2019)105号]
贵州省教育厅自然科学研究项目[黔教合KY(2015)476号]。
关键词
毛钩藤
炭疽病
博宁炭疽菌
生物学特性
药剂筛选
Uncaria hirsuta Havil.
Anthracnose
Colletotrichum boninense
Biological characteristics
Fungicide screening
作者简介
邓洁(1984-),男,硕士,实验师,研究方向:植物保护,E-mail:61076191@qq.com;通讯作者:龙友华,E-mail:gzlyh126@126.com。
