摘要
以6月龄肉桂实生苗为实验材料,采用聚乙二醇(PEG 6000)模拟干旱胁迫,对不同干旱程度下种苗的生理指标(渗透物质、抗氧化酶等)和化学成分以及两者的相关性进行了研究。结果表明:叶片中叶绿素含量和相对含水量随着PGE 6000(0,5%,10%,15%)和时间(3,5,7 d)的增加逐渐下降,可溶性蛋白含量、可溶性糖含量和过氧化氢酶(CAT)活性呈上升趋势,但随着时间的推移上升速率减缓;过氧化物酶(POD)和超氧化物歧化酶(SOD)活性、丙二醛(MDA)和脯氨酸含量呈先升高后下降趋势;香豆素、肉桂醛、肉桂酸和二甲氧基肉桂醛的含量呈下降趋势,肉桂醇含量持续上升。干旱胁迫下肉桂幼苗根系活性氧和NO含量荧光信号明显强于对照。进一步相关性分析表明,香豆素含量、二甲氧基肉桂醛含量与渗透调节物质含量呈显著负相关(P<0.05),肉桂酸含量与POD,SOD活性呈极显著负相关(P<0.01)。研究发现肉桂幼苗在短期或轻度干旱胁迫下表现出一定的耐旱潜力,但干旱超过一定程度,幼苗体内生理代谢会失衡。
Six month old Cinnamomum cassia seedlings were used to simulate drought stress with polyethylene glycol(PEG 6000). The physiological indicators(osmotic substances, antioxidant enzymes, etc.) and chemical components of seedlings under different drought levels and the correlation between the two were studied. The results showed that the chlorophyll content and relative water content decreased gradually with the increase of PGE 6000(0, 5%, 10%, 15%) concentration and time(3, 5, 7 d), while the soluble protein content, soluble sugar content and catalase(CAT) activity increased, but the rising rate slowed down with the time. The activities of peroxidase(POD), superoxide dismutase(SOD), malondialdehyde(MDA) and proline content increased at first and then decreased. The content of coumarin, cinnamaldehyde, cinnamic acid and dimethoxycinnamaldehyde decreased, while the content of cinnamyl alcohol continued to increase.Under drought stress, the fluorescence signals of reactive oxygen species and no contents in roots of C. cassia seedlings were significantly stronger than those of the control.Further correlation analysis showed that coumarin content, di-methoxycinnamaldehyde content and osmoregulation substance content were significantly negatively correlated(P<0.05), cinnamic acid content was significantly negatively correlated with POD and SOD activities(P<0.01).It was found that C. cassia seedlings showed a certain degree of drought tolerance under short-term or mild drought stress, but if the drought exceeded a certain degree, the physiological metabolism of the seedlings would be unbalanced.
作者
钟磊
廖沛然
刘长征
钱家萍
何婉聪
罗碧
杨全
ZHONG Lei;LIAO Pei-ran;LIU Chang-zheng;QIAN Jia-ping;HE Wan-cong;LUO Bi;YANG Quan(Guangdong Provincial Southern Medicine Standardized Cultivation and Comprehensive Development Engineering Technology Research Center,Guangzhou Comprehensive Test Station of National Traditional Chinese Medicine Industry Technology System,the Key Laboratory of Lingnan Medicinal Materials Production and Development of the State Administration of Traditional Chinese Medicine,School of Traditional Chinese Medicine,Guangdong Pharmaceutical University,Guangzhou 510006,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2021年第9期2158-2166,共9页
China Journal of Chinese Materia Medica
基金
国家“重大新药创制”科技重大专项(2018ZX09721004-001)
广东特支计划项目(2016TQ03R510)
罗定肉桂产业发展基础研究项目(2018-082)
肉桂种子质量分级标准及种苗繁育技术研究项目(2020-092)。
关键词
肉桂
干旱胁迫
生理生化
化学成分
Cinnamomum cassia
drought stress
physiological and biochemical
chemical composition
作者简介
钟磊,硕士研究生,主要从事中药资源开发与品质评价研究,Tel:(020)39252353,E-mail:1045124923@qq.com;通信作者:杨全,教授,主要从事道地药材道地性机制研究,E-mail:yangquan7208@vip.163.com。
