摘要
目的建立2型糖尿病气阴两虚型病证结合动物模型及评价指标。方法SD大鼠48只随机分为空白组、病证模型组和高糖高脂组,空白组喂饲普通饲料,其余两组喂饲高脂饲料。饲养4周后,病证模型组大鼠禁食12 h腹腔注射链脲佐菌素(STZ)40 mg/kg,1周后测定大鼠空腹血糖值大于11.1 mmol/L视为糖尿病造模成功,继续饲养至14周试验结束。监测大鼠体征变化及各项实验室指标。结果与空白组比较,病证模型组大鼠第8~14周精神、运动评分均显著升高(P<0.01),体重从第4周开始显著降低(P<0.05或P<0.01),进食量和饮水量均从第8周开始显著增加(P<0.05或P<0.01),抓力从第12周开始显著降低(P<0.01),第6~14周的随机血糖、空腹血糖均显著升高(P<0.01),尿糖检测结果均显著升高(P<0.01),呼吸频率、脉搏幅度、舌象值、PT、APTT、胸腺指数、CD4、CD4/CD8值均显著降低(P<0.05或P<0.01),TC、TG、LDL-C、cAMP、cAMP/cGMP值均显著升高(P<0.05或P<0.01),病理结果显示胰岛组织损伤严重,功能明显下降。结论采用高糖高脂喂养大鼠4周后腹腔注射STZ 40 mg/kg,继续饲养至14周可制备2型糖尿病气阴两虚病证结合动物模型,通过对大鼠进食量、饮水量的测定,运动、精神状态的评分,抓力、呼吸、脉搏、舌象等体征信息的采集,PT、APTT、TC、TG、LDL-C、cAMP、cAMP/cGMP、CD4、CD4/CD8及胸腺指数等指标的检测,结合胰腺组织的病理改变等可较好的反映该模型的证候特点。
Objective To establish an animal model of type 2 diabetes with deficiency of Qi and Yin,and its evaluation index.Methods Forty-eight Sprague Dawley rats were randomly divided into blank,disease and syndrome model,and high sugar and fat groups.The blank group was fed an ordinary diet,whereas the other two groups were fed a high fat diet.After 4 weeks of feeding,rats in the disease model group were fasted for 12 h and then injected with 40 mg/kg streptozotocin intraperitoneally.After 1 week,the fasting blood glucose level of the rats was measured,and a fasting blood glucose level of>11.1 mmol/L was considered to be successful modeling of diabetes.The rats were continued to be fed until the end of the experiment at 14 weeks.Changes in rat physical signs and various laboratory indexes were monitored.Results Compared with those of rats in the blank group,the mental and motor scores of rats in the disease and syndrome model group were increased significantly at weeks 8~14(P<0.01).The weight of rats was decreased significantly from week 4(P<0.05 or P<0.01),food intake and water intake were increased significantly from week 8(P<0.05 or P<0.01),and grip strength was significantly decreased from week 12(P<0.01).At weeks 6~14,both random and fasting blood glucose were increased significantly,and urine sugar was increased significantly(P<0.01).The respiratory rate,pulse amplitude,and tongue values,PT,APTT,thymus index,and CD4 and CD4/CD8 values were reduced significantly(P<0.05 or P<0.01).TC,TG,LDL-C,cAMP,and cAMP/cGMP values were increased significantly(P<0.05 or P<0.01).Pathological result showed that islet tissue was seriously damaged,and its function was reduced significantly.Conclusions Rats fed with high glucose and fat for 4 weeks were intraperitoneally injected with 40 mg/kg streptozotocin and further fed with high glucose and fat for 14 weeks to prepare an animal model of type 2 diabetes combined with Qi and Yin deficiency.Food intake,water intake,movement score,mental state score,grasping ability,respiration,pulse,tongue image and other information were collected.PT,APTT,TC,TG,LDL-C,cAMP,cAMP/cGMP,CD4,CD4/CD8 and thymus index were detected.Combined with pathological changes of pancreatic tissue,the syndrome characteristics of this model could be well reflected.
作者
张译心
王鑫
刘博
纪凤兰
温富春
丁涛
徐惠波
ZHANG Yixin;WANG Xin;LIU Bo;JI Fenglan;WEN Fuchun;DING Tao;XU Huibo(Jilin Academy of Chinese Medicine Sciences,Changchun 130012,China;Changchun University of Traditional Chinese Medicine,Changchun 130117)
出处
《中国实验动物学报》
CAS
CSCD
北大核心
2021年第2期219-229,共11页
Acta Laboratorium Animalis Scientia Sinica
基金
国家重大新药创制专项(2018ZX09711001-009-005)。
关键词
糖尿病
气阴两虚
病证结合
体征
模型
diabetes
Qi and Yin deficiency
combination of disease and syndrome
characterization
model
作者简介
张译心(1994-),女,在读硕士研究生,研究方向:动物模型的制备。Email:zyx_1942@163.com;通信作者:徐惠波(1963-),女,主任药师,硕士生导师,研究方向:中药基础及应用开发。Email:xhb_6505@163.com。
