摘要
目的:以富硒大米中不同溶解性的硒蛋白为研究对象,研究了其理化特性和抗氧化活性。方法:采用Osborne分级分离法从富硒大米中制备水溶性硒蛋白(WSP)、盐溶性硒蛋白(SSP)、醇溶性硒蛋白(ESP)和碱溶性硒蛋白(ASP),对比其蛋白含量、总硒含量和硒蛋白的亚基分子量分布,通过紫外、红外光谱分析确定其特征官能团,并采用液相色谱-质谱联用法分析其硒代氨基酸组成等理化特性;在此基础上进一步分析了各类大米硒蛋白的抗氧化活性。结果:在4种不同溶解性的大米硒蛋白中,ASP具有最高的蛋白含量和硒含量(P<0.05),且以小分子蛋白为主。ASP中存在3种硒代氨基酸,包括硒代半胱氨酸(300.22±6.88μg/kg)、甲基硒代半胱氨酸(170.19±2.87μg/kg)及少量硒代甲硫氨酸。大米硒蛋白的总抗氧化能力(T-AOC)、DPPH自由基清除能力与样品浓度呈良好的量效关系;在相同浓度下,不同大米硒蛋白的T-AOC、DPPH自由基清除能力的强弱顺序均为ASP>WSP>SSP。通过抗氧化活性与硒含量之间的相关性分析,两者之间存在极显著的正相关性(P<0.01)。结论:ASP是进一步纯化和研究较为理想的富硒大米提取物。
Objective:Different kinds of Se-proteins from selenium-enriched rice were used as raw materials to analyze their physicochemical properties and antioxidant activities.Methods:Water-soluble proteins(WSP),salt-soluble proteins(SSP),ethanol-soluble proteins(ESP)and alkali-soluble proteins(ASP)were prepared by Osborne classification method.The protein content and selenium content of Se-proteins,as well as the molecular weight distribution of Se-proteins were determined.The characteristic groups were conducted by ultraviolet(UV)spectrum and Fourier transform infrared(FT-IR)spectroscopy.The composition of seleno-amino acids in ASP from selenium-enriched rice was performed by liquid chromatography-mass spectrometry(LC-MS)method.On this basis,the total antioxidant capacity(T-AOC)and DPPH free radical scavenging capacity of Se-proteins were further analyzed.Results:Among all Se-proteins,ASP had maximum protein and selenium contents(P<0.05).Three Se-amino acids in ASP from selenium-enriched rice were identified and determined,including selenocysteine(300.22±6.88μg/kg)and methylselenocysteine(170.19±2.87μg/kg),as well as a small quantity of selenomethionine.There were obvious dose-response relationships between three Se-proteins and antioxidant capacity.ASP possessed the highest T-AOC and DPPH radical scavenging capacity among all Se-proteins.Through the correlation analysis between the antioxidant activity and the selenium content of Se-proteins,there were extremely significant positive correlation(P<0.01).Conclusion:ASP as selenium-enriched rice extract could be the most ideal protein to be further purified and studied.
作者
唐雅园
何雪梅
孙健
游向荣
李昌宝
刘国明
盛金凤
李丽
周葵
易萍
韦珍
零东宁
唐杰
TANG Yayuan;HE Xuemei;SUN Jian;YOU Xiangrong;LI Changbao;LIU Guoming;SHENG Jinfeng;LI Li;ZHOU Kui;YI Ping;WEI Zhen;LING Dongning;TANG Jie(Agro-food Science and Technology Research Institute,Guangxi Academy of Agricultural Sciences,Guangxi Key Laboratory of Fruits and Vegetables Storage-processing Technology,Nanning 530007,China)
出处
《食品工业科技》
CAS
北大核心
2021年第9期44-50,共7页
Science and Technology of Food Industry
基金
广西科技重大专项(桂科AA17202010、AA17202029)
广西“八桂学者”专项经费([2016]21)
科技先锋队“强农富民”“六个一”专项行动项目(桂农科盟202015)
广西农业科学院基本科研业务费项目(桂农科2021YTI16,2021YT113、2020YM40)。
关键词
富硒大米
硒蛋白
理化特性
抗氧化活性
selenium-enriched rice
Se-proteins
physicochemical properties
antioxidant activities
作者简介
并列第一作者:唐雅园(1991-),女,硕士,助理研究员,研究方向:食品营养,E-mail:tangyayuan@gxaas.net。;并列第一作者:何雪梅(1981-),女,硕士,副研究员,研究方向:天然产物化学,E-mail:xuemeihegx@gxaas.net。;通信作者:孙健(1978-),男,博士,二级研究员,研究方向:农产品加工,E-mail:jiansun@gxaas.net。
