摘要
目的通过谱效相关法探究酒炙丹参增强抗凝血活性的物质基础。方法采用HPLC法建立丹参生品与酒炙品指纹图谱,并进行相似度评价。测定不同批丹参生品与酒炙品的体外抗凝血效价,包括活化部分凝血活酶时间(APTT)、凝血酶原时间(PT)、凝血酶时间(TT)和纤维蛋白原(FIB)含量。以丹参生品与酒炙品的HPLC图谱的峰面积及其抗凝血效价为基础,采用偏最小二乘判别分析(PLS-DA)法对HPLC图谱和抗凝血效价进行相关性分析。结果10批生丹参饮片HPLC指纹图谱相似度为0.991~0.999,10批酒丹参饮片与对照图谱的相似度为0.982~0.999。丹参酒炙后抗凝血活性显著增强。PLS-DA法分析得出,指纹图谱中7(丹酚酸B)、6(紫草酸)、5(迷迭香酸)、15(VIP均大于1)号峰是导致丹参酒炙后抗凝血活性增强的主要成分。结论该方法能较精准地找到丹参酒炙增强抗凝血活性的主要贡献成分。
AIM To explore the spectrum-effect correlation between anticoagulant activity enhancement and ingredients of wine-processed Salvia miltiorrhiza Bge..METHODS Crude and wine-processed products of S.miltiorrhiza were subjected to fingerprints determination and similarity evaluation by HPLC method,and assessment of in vitro anticoagulant potency,including APTT,PT,TT and FIB.Based on the peak area and anticoagulant potency of the HPLC profiles of S.miltiorrhiza,PLS-DA method was performed to analyze the correlation of the HPLC profile and anticoagulant potency.RESULTS In contrast to the 10 batches of raw S.miltiorrhiza sharing 0.991-0.999 similarity in HPLC fingerprints,10 batches wine-processed products displayed 0.982-0.999 similarity,indicating a significantly enhanced anticoagulant activity.PLS-DA analysis revealed the main components,the peaks 7(salvianolic acid B),6(shikonic acid),5(rosmarinic acid),15(VIP values greater than 1)in the fingerprints conferring enhanced anticoagulant activity.CONCLUSION The spectrum-effect correlation-based study can more accurately reveal the main components of wine-processed S.miltiorrhiza contributing enhanced anticoagulant activity.
作者
周巧
张智慧
张学兰
王悦
甄臻
奚亚亚
ZHOU Qiao;ZHANG Zhi-hui;ZHANG Xue-lan;WANG Yue;ZHEN Zhen;XI Ya-ya(College of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250355,China;Shandong Provincial University Traditional Chinese Medicine Quality Control and Construction of Whole Industry Chain Collaborative Innovation Center,Jinan 250355,China)
出处
《中成药》
CAS
CSCD
北大核心
2021年第4期954-958,共5页
Chinese Traditional Patent Medicine
基金
国家重点研发计划课题(2018YFC1707002)
国家自然科学基金项目(81973486)
山东省重点研发计划项目(2017GSF19113)
山东省中医药科技发展计划项目(2019-0955)。
关键词
丹参
酒炙
指纹图谱
抗凝血活性
谱效相关
PLS-DA
Salvia miltiorrhiza Bge.
wine processing
fingerprints
anticoagulant effect
spectral-effect correlation
PLS-DA
作者简介
周巧(1996-),女,硕士生,研究方向为中药饮片制备技术与质量评价。Tel:15964557112,E-mail:z452710682@163.com;通信作者:张学兰(1963-),女,硕士,教授,研究方向为中药炮制原理与饮片质量控制。Tel:(0531)89628081,E-mail:zhang8832440@sina.com。
