摘要
木霉菌能够促进植物对盐胁迫的耐受性,研究选用耐盐木霉选择培养基筛选获得的木霉菌株ST02,对其耐盐活性进行检测,具有较强耐盐活性。用ST02木霉菌株的孢子悬液对番茄种子和幼苗进行处理,分析木霉处理对盐胁迫条件下番茄种子萌发,幼苗生长和耐盐生理生化的影响。结果显示:ST02孢子悬液促进NaCl胁迫下番茄种子的发芽率和发芽势,提高200 mmol/L NaCl胁迫下番茄幼苗的存活率、株高和鲜重,以及番茄幼苗的叶绿素含量和净光合速率,影响番茄幼苗的离子渗漏率和丙二醛(malondialdehyde, MDA)含量,提高超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)等抗氧化酶的活性。说明木霉ST02孢子悬液处理能够通过提高植物的抗氧化防御反应促进番茄对NaCl胁迫的耐受性。
It has shown that Trichoderma spp.can promote plant tolerance to salt stresses.Trichoderma strain ST02 which isolated by using salt-tolerant selection medium was selected.Its salt-tolerant activity was detected with strong salt-tolerant activity.The tomato seeds and seedlings were treated with spore suspension of ST02,and the effects of Trichoderma treatment on tomato seed germination,seedling growth and salt-tolerance physiology and biochemistry under salt stress were analyzed.The results show that ST02 spore suspension promotes germination rate and potential of tomato seeds under NaCl stresses.It increases the survival rate,plant height and fresh weight of tomato seedlings,as well as the chlorophyll content and net photosynthetic rate of tomato seedlings under 200 mmol/L NaCl stress.It also affects the ion leakage rate and malondialdehyde(MDA)content of tomato seedlings,increases the activity of antioxidant enzymes including superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT).It indicates that the treatment with Trichoderma ST02 spore suspension can promote the tolerance of tomato to NaCl stress by improving the antioxidant defense reaction of plants.
作者
赵忠娟
扈进冬
陈凯
魏艳丽
李纪顺
ZHAO Zhong-juan;HU Jin-dong;CHEN Kai;WEI Yan-li;LI Ji-shun(Ecology Institute of Shandong Academy of Sciences,Qilu University of Technology(Shandong Academy of Sciences),Jinan 250103,China;Shandong Province Key Laboratory of Applied Mocrobiology,Jinan 250103,China)
出处
《科学技术与工程》
北大核心
2021年第7期2632-2639,共8页
Science Technology and Engineering
基金
山东省自然科学基金(ZR2019BC076,ZR2017BC075)
山东省重大科技创新工程(2019JZZY010723)
山东省科学院院地产学研协同创新基金(2019-CXY5)。
关键词
耐盐木霉
种子发芽
幼苗生长
耐盐生理生化
抗氧化防御
salt-tolerant Trichoderma
seed germination
seedling growth
salt-tolerance physiology and biochemistry
antioxidant response
作者简介
第一作者:赵忠娟(1986-),女,汉族,山东潍坊人,博士研究生,副研究员。研究方向:植物-微生物相互作用及对盐渍化土地修复。E-mail:zzjfrances@aliyun.com;通信作者:李纪顺(1971-),男,汉族,山东临沂人,应用研究员。研究方向:应用微生物(生防、促生、环境修复等)。E-mail:yewu2@sdas.org。
