摘要
目的探索结肠腺瘤患者和健康人肠液菌群结构的差异性,并找到特定的差异分类。方法选取2019年1月至2019月9年上海电力医院消化内科结肠腺瘤患者10例(腺瘤组)和健康人12例(对照组),内镜下取其肠液样本,对基因组DNA完成精准提取,对细菌16SrRNA基因序列所属的V3-V4高变片段进行系统的PCR扩增,对PCR产物加以检测,将纯化后的混合DNA产物于实验用lluminaMiSeq平台放置,进行高通量测序操作。结果两组之间微生物群OTU(Operational Taxonomic Unit,可操作分类单元)分布存在明显差异。腺瘤组变形菌门水平(33.98%)显著高于对照组(17.31%),P<0.05,腺瘤组厚壁菌门水平(36.29%)显著低于对照组(46.5%),P<0.05。腺瘤组假单胞菌属、杆菌属丰度显著增加(P<0.05)。结论结肠腺瘤患者肠液菌群结构呈明显改变,形成了易于腺瘤生长的肠道微生态环境。
Objective To explore the structural differences of intestinal fluid flora between healthy people and patients with nodular tumors,and to find specific differences in classification.Methods From January 2019 to September 2019,10 patients with colonic adenoma(adenoma group)and 12 healthy people(control group)from the Department of Gastroenterology,Shanghai Electric Power Hospital,were collected from January 2019 to September 2019.Intestinal juice samples were collected under endoscopy,and genomic DNA was accurately extracted,Perform systematic PCR amplification on the V3-V4 hypervariable fragments of the bacterial 16SrRNA gene sequence,detect the PCR products,and place the purified mixed DNA products on the experimental lluminaMiSeq platform for high-throughput sequencing operations.Results Compared with the control group,the composition of the intestinal fluid flora of the adenoma group was significantly different(P<0.01).At the phylum level:the level of Proteobacteria in patients with colon adenoma increased(P<0.05),and the level of Firmicutes decreased.At the genus level:Pseudomonas and Bacillus in patients with colon adenoma increased(P<0.05).Conclusion The structure of the intestinal fluid flora of patients with colon adenoma has changed significantly,forming an intestinal micro-ecological environment that is prone to adenoma growth.
作者
张蓓
陈文晖
汪妍
ZHANG Bei;CHEN Wen-hui;WANG Yan(Department of Gastroenterology, Shanghai Electric Power Hospital, Shanghai 202157PPPPP)
出处
《现代消化及介入诊疗》
2021年第1期56-60,共5页
Modern Interventional Diagnosis and Treatment in Gastroenterology
基金
上海电力医院院级科研课题项目(2019-04)。
关键词
结肠腺瘤
肠道菌群
差异分析
Colonic adenoma
Intestinal flora
Difference analysis
作者简介
通讯作者:汪妍,E-mail:wy857cc@163.com。
