摘要
目的探究脊柱结核患者血清特异性循环miRNA表达水平及其临床意义。方法选择2016年6月至2018年9月深圳市第三人民医院收治的50例脊柱结核患者作为观察组,选择同期在上述医院进行体检的50例健康志愿者作为对照组。分别提取其外周血血清中RNA,经miRNA基因芯片检测筛选出差异表达的miRNA,采用实时荧光定量PCR进行验证。比较两组受检者及观察组患者手术前后的miRNA表达水平。结果经miRNA基因芯片初步筛选出17个差异表达的miRNA,其中16个表达下调,1个表达上调;经实时荧光定量PCR验证,hsa-miR-30c-5p、hsa-miR-432-5p、hsa-miR-192-5p、hsa-miR-532-5p的表达量显著下调,hsa-miR-210-3p的表达量显著上调;观察组患者术前的hsa-miR-30c-5p、hsa-miR-432-5p、hsa-miR-192-5p、hsa-miR-532-5p表达水平分别为1.48±0.45、1.67±0.32、1.84±0.43、1.49±0.35,明显低于对照组的11.35±3.08、4.72±1.13、5.29±1.49、4.11±1.28,hsa-miR-210-3p表达水平为3.92±1.34,明显高于对照组的1.28±0.31,组间比较差异均有显著统计学意义(P<0.01);观察组患者术后1年的hsa-miR-30c-5p、hsa-miR-432-5p、hsa-miR-192-5p、hsa-miR-532-5p表达水平分别为10.31±2.29、4.01±1.22、4.69±1.17、3.52±0.87,明显高于术前的1.48±0.45、1.67±0.32、1.84±0.43、1.49±0.35,hsa-miR-210-3p表达水平为1.86±0.47,明显低于术前的3.92±1.34,组间比较差异均有统计学意义(P<0.05);hsa-miR-30c-5p在诊断脊柱结核上的敏感度、特异度、准确度、阳性预测值和阴性预测值分别为100.00%、96.00%、98.00%、96.15%和100.00%,明显高于hsa-miR-432-5p、hsa-miR-192-5p、hsa-miR-532-5p和hsa-miR-210-3p,差异均有统计学意义(P<0.05)。结论脊柱结核患者的血清中存在特异性表达的循环miRNAs,其检测对于脊柱结核的临床诊断具有一定的临床参考价值。
Objective To explore the expression of serum specific circulating miRNA in patients with spinal tuberculosis and its clinical significance.Methods From June 2016 to September 2018,50 patients with spinal tuberculosis admitted to the Third People's Hospital of Shenzhen were selected as the observation group,and 50 healthy volunteers who had physical examination in our hospital at the same time were selected as the control group.RNA was extracted from the peripheral blood serum,and the miRNA was screened by miRNA microarray.The real-time fluorescent quantitative PCR was used for verification.The miRNA expression of the two groups before and after operation was compared.Results A total of 17 miRNAs with different expression were screened by miRNA microarray,16 of them were down-regulated and 1 was up-regulated.The expression of hsa-miR-30c-5p,hsa-miR-432-5p,hsa-miR-192-5p,and hsa-miR-532-5p were down-regulated and that of hsa-mir-210-3p was up-regulated.The expressions of hsa-miR-30c-5p,hsa-miR-432-5p,hsa-miR-192-5p,and hsa-miR-532-5p in the observation group were 1.48±0.45,1.67±0.32,1.84±0.43,1.49±0.35,respectively,which were significantly lower than corresponding 11.35±3.08,4.72±1.13,5.29±1.49,4.11±1.28 in the control group,and the expression of hsa-miR-210-3p in the observation group was 3.92±1.34,which was significantly higher than 1.28±0.31 in the control group(all P<0.05).The expressions of hsa-miR-30c-5p,hsa-miR-432-5p,hsa-miR-192-5p and hsa-miR-532-5p in the observation group after 1 year were 10.31±2.29,4.01±1.22,4.69±1.17,3.52±0.87,which were significantly higher than corresponding 1.48±0.45,1.67±0.32,1.84±0.43,1.49±0.35 before operation,while the expression of hsa-miR-210-3p was 1.86±0.47,which was significantly lower than 3.92±1.34 before operation(all P<0.05).The sensitivity,specificity,accuracy,positive predictive value,and negative predictive value of hsa-miR-30c-5p in the diagnosis of spinal tuberculosis were 100.00%,96.00%,98.00%,96.15%,and 100.00%,respectively,which were significantly higher than those of hsa-miR-432-5p,hsa-miR-192-5p,hsa-miR-532-5p,and hsa-miR-210-3p(all P<0.05).Conclusion There are circulating miRNAs with specific expression in the serum of patients with spinal tuberculosis.The detection of miRNAs has certain clinical reference value for the clinical diagnosis of spinal tuberculosis.
作者
黄志刚
马克
刘晶
HUANG Zhi-gang;MA Ke;LIU Jing(Department of Orthopedics,the Third People's Hospital of Shenzhen,Shenzhen 518100,Guangdong,CHINA)
出处
《海南医学》
CAS
2021年第3期297-300,共4页
Hainan Medical Journal
基金
广东省深圳市科技计划项目(编号:JCYJ20160427152847715)。
关键词
脊柱结核
循环微小RNA
实时荧光定量PCR
聚类分析热图
诊断标志物
Spinal tuberculosis
Circulating microRNA
Real-time fluorescence quantitative PCR
Cluster analysis thermogram
Diagnostic markers
作者简介
通讯作者:黄志刚,E-mail:hzguanlyo@126.com。
