摘要
[目的]从发酵酸鱼中筛选鉴定得到一株产植物乳杆菌素的植物乳杆菌MMB-03,通过研究植物乳杆菌素的酸、温度稳定性及抑菌效果,确定了该植物乳杆菌素的抑菌特性。[方法]采用形态学、生理生化、16S rDNA分析鉴定该菌株为植物乳杆菌。采用打孔法测定植物乳杆菌素的抑菌效果。[结果]该植物乳杆菌素在pH 4.0~7.0时均有较强的抑菌活性;121℃处理20 min仍有良好的抑菌活性;对指示菌金黄色葡萄球菌、大肠杆菌、蜡样芽孢杆菌等均有良好的抑菌效果。[结论]植物乳杆菌MMB-03在水产饲料和食品保藏方面具有良好的应用前景。
[Objective]A strain of Lactobacillus plantarum MMB-03 was obtained from fermented acid fish.The antibacterial properties of plantaricin from Lactobacillus plantarum MMB-03 were determined by researching acid stability,temperature stablility,and bacteriostatic activity.[Method]The strain was identified as Lactobacillus plantarum by morphology,hysiological and biochemical and 16S rDNA.The bacteriostatic effect of plantaricin was determined by punching.[Result]The plantaricin had strong bacteriostatic activity at pH 4.0-7.0,and had good bacteriostatic activity at 121℃ for 20 min.It also had good bacteriostatic effect on indicator bacteria Staphylococcus aureus,Escherichia coli and Bacillus cereus.[Conclusion]Lactobacillus plantarum MMB-03 has a good application prospect in aquatic feed and food preservation.
作者
赵永慧
杨杰
朱卿正
郭长明
柴金龙
王淑军
房耀维
盘赛昆
ZHAO Yong-hui;YANG Jie;ZHU Qing-zheng(Jiangsu Ocean University,Lianyungang,Jiangsu 222061)
出处
《安徽农业科学》
CAS
2020年第17期189-192,196,共5页
Journal of Anhui Agricultural Sciences
基金
江苏省兽用生物制药高技术研究重点实验室开放课题(NSFK201803)
江苏省研究生科研与实践创新计划项目(SJCX19_0958)。
关键词
植物乳杆菌
鉴定
细菌素
理化性质
抑菌
Lactobacillus plantarum
Appraisal
Bacteriocins
Physical and chemical properties
Bacteriostatic
作者简介
赵永慧(1994-),女,山东潍坊人,硕士研究生,研究方向:食品加工与安全;通信作者:盘赛昆,副教授,博士,硕士生导师,从事食品加工与安全研究。
