摘要
以致病弧菌T1、T2为检测菌,采用平板抑菌圈检测法,研究乳酸杆菌L1及其代谢产物的抑菌能力。结果表明:L1发酵上清液对两种弧菌的抑制效果和发酵液对弧菌的抑菌作用相似,即对弧菌T1的抑制作用强于T2;L1 发酵液对弧菌T1、T2的抑制能力强于上清液,表明乳酸杆菌及其代谢物质对弧菌具有协同抑制作用;L1的发酵液经3倍稀释仍对弧菌T1有抑制作用;乳酸杆菌L1生长不同时期的代谢产物对弧菌的抑菌活性不同,代谢产物的抑菌能力在衰退期>稳定期>生长期;L1的发酵液经沸水浴处理15 min其抑菌活性不变,表明抑菌代谢物质具有较好的耐热性。
The inhibitory effects of Lactobacillaceae LI and its metabolic product on pathogen Vibrio sp. TI ,T2 were examined by a zone plate inhibitory method. The results show that the antibacterial activity in supernatant liquid of Lactobacillaceae to T1 and T2 is similar to that of fermentation liqud,but the inhibitory effect of L1 on Vibrio T1 is stronger than that on T2. Fermentation liquid has a higher antibacterial activity than supernatant liquid, indicates that Lactobacillaceae and its metabolic product have a conjunct inhibitory effect on Vibrio TI and T2. The antibacterial activity of fermentation liquid still remains after 3 times dilution. The antibacterial activity of meta- bolic product of lactobacillaceae to Vibrio T1 and T2 vary from different growth periods, with being stronger in the decline period 〉 stability period 〉 growth period. The inhibitory activity of fermentation liquid of LI treated with boiled water for 15 min is invariable, shows that temperature tolerance of the inhibitory substance is better.
出处
《南方水产》
2005年第1期62-65,共4页
South China Fisheries Science
基金
水科院重点科研项目(2001-4-2)
广东省重大科技专项(A3050304)
关键词
乳酸杆菌
弧菌
抑菌能力
Lactobacillaceae
Vibrio sp.
antibacterial activity
作者简介
杨莺莺（1963-），女，副研究员，主要从事水产动物益生素的研究。E-mail：yyy402@126．com
