摘要
目的:探讨二氢丹参酮经糖原合成酶激酶3β(GSK3β)/Wnt途径调节膀胱癌T-24细胞增殖及侵袭的机制。方法:体外培养膀胱癌T-24细胞,选择对数生长期细胞进行实验。实验分为对照组、阳性组、二氢丹参酮低、中、高剂量组。对照组不进行处理,阳性组给予终浓度为1mg·L^-1的吉西他滨,二氢丹参酮低、中、高剂量组分别给予终浓度为10,20,40 mg·L^-1的二氢丹参酮,继续培养24 h后检测细胞增殖率、细胞体外侵袭能力、细胞凋亡和细胞周期,用实时荧光定量PCR(RT-PCR)检测GSK3β、β-连环蛋白(β-catenin)、细胞周期蛋白D1(Cyclin D1)、E-钙黏蛋白(E-cadherin)和波形蛋白(Vimentin)mRNA表达。结果:与对照组比较,阳性组和二氢丹参酮各剂量组的T-24细胞增殖率、侵袭细胞数、β-catenin、Cyclin D1和Vimentin mRNA表达降低(P<0.05),细胞凋亡率、S期细胞数、GSK3β、E-cadherin mRNA表达增加(P<0.05),且二氢丹参酮各剂量组呈剂量依赖性,但效果不如阳性组(P<0.05)。结论:二氢丹参酮可以抑制膀胱癌T-24细胞增殖和侵袭,其机制可能与激活GSK3β/Wnt途径有关。
Objective:To investigate the mechanisms of dihydrotanshinone in regulating the proliferation and invasion of bladder cancer T-24 cells via GSK3β/Wnt pathway.Methods:Bladder cancer T-24 cells were cultured in vitro,and logarithmic growth phase cells were selected for the experiment and divided into the control group,the positive group,and low,medium and high dose groups of dihydrotanshinone.The control group was without any treatment.The positive group was given gemcitabine at the final concentration of1 mg·L^-1.The low,medium and high dose group of dihydrotanshinone was given dihydrotanshinone at the final concentration of 10,20 and 40 mg·L^-1,respectively.After 24 hours of continuous culture,the cell proliferation rate,cell invasion ability in vitro,cell apoptosis and cell cycle were detected.Real-time fluorescence quantitative PCR(RT-PCR)was used to detect the expressions of GSK3β,β-catenin,Cyclin D1,E-cadherin and Vimentin mRNA.Results:Compared with those in the control group,the T-24 cell proliferation rate,invasive cell number,β-catenin,Cyclin D1 and Vimentin mRNA in the positive group and all dihydrotanshinone groups were significantly decreased(P<0.05),and the apoptotic rate,S-phase cell number,GSK3βand E-cadherin mRNA increased(P<0.05)in a dose-dependent manner in the three dihydrotanshinone groups,while the effects were not as good as those of the positive group(P<0.05).Conclusion:Dihydrotanshinone can inhibit the proliferation and invasion of bladder cancer T-24 cells,which may be related to the activation of GSK3β/Wnt pathway.
作者
倪建华
朱江波
殷国林
章庆华
Ni Jianhua;Zhu Jiangbo;Yin Guolin;Zhang Qinghua(Department of Urology,Taizhou First People's Hospital,Zhejiang Taizhou 318020,China)
出处
《中国药师》
CAS
2020年第7期1256-1260,共5页
China Pharmacist
作者简介
通讯作者:倪建华,Tel:18205767801,E-mail:jianhuani6843@163.com。
