摘要
确定绿茶末中L-茶氨酸纯化的最佳离子交换树脂类型、上样pH值、氨水洗脱浓度。绿茶末经过提取、大孔树脂除杂后进行离子交换树脂纯化,考查离子树脂类型、上样pH值、氨水洗脱浓度对L-茶氨酸纯化效果的影响。结果表明,4种型号离子交换树脂对L-茶氨酸纯化效果差别较大,不同上样pH值、不同浓度氨水洗脱对L-茶氨酸的纯化效果存在差异。LSI-010 (H+)对L-茶氨酸的纯化效果最好,并确定了上样pH值为5.0~5.5,氨水洗脱浓度为0.5 mol/L。
To select the optimal type of ion exchange resin,loading pH,and elution of ammonia concentration for the purification of theanine from green tea dust.Ion exchange resin was used for purifying theaine,which has been extracted and then separated by the macroporous resin.It was studied on the effects of ion resin type,loading pH and elution of ammonia concentration.The results showed the four types of ion exchange resin has aquite different effect on purifying theanine in green tea dust.The difference also exist in loading pH and ammonia concentrations.LSI-010(H+)was the best choice on theanine purification in green tea dust,and the optimum loading pH was 5.0~5.5,and the ammonia concentration was 0.5 mol/L.
作者
刘晶晶
陈志元
王玉
江丹
李名洁
LIU Jingjing;CHEN Zhiyuan;WANG Yu;JIANG Dan;LI Mingjie(Jing Brand Chizhengtang Pharmaceutical Co.,Ltd.,Huangshi,Hubei 435000,China)
出处
《农产品加工》
2020年第7期14-16,共3页
Farm Products Processing
关键词
绿茶末
L-茶氨酸
离子交换树脂
纯化
green tea dust
L-theanine
ion exchange resin
purification
作者简介
刘晶晶(1993-),女,硕士,助理工程师,研究方向为食品工程;通讯作者:陈志元(1976-),男,本科,高级工程师,研究方向为中药现代技术。
