摘要
L-茶氨酸是茶叶中游离氨基酸的主要组成部分,关于其良好的生理活性已有广泛报道。首次报道了来源于Cunnighamell aechinulata9980的L-氨基酰化酶用于高光学纯度的L-茶氨酸的酶法制备。该酶在pH6.5,底物N-乙酰-DL-茶氨酸浓度为50mmol/L,且有40mmol/LCoCl2时催化效果较好。结果表明,在上述条件下,50℃作用12h得L-茶氨酸22.5mmol/L,转化率90%。
L-theanine （ γ-glutamylethylamide ） is the main free amino acid component of tea and its favorable physiological effects on mammals have been reported. An enzymatic method for optically pure L-theanine production with a new L-aminoacylases-production fungi Cunnighamella echintdata 9980 was developed. The optimum conditions for enzymatic catalysis were at pH 6.5 with 50 mmol/L N-Acyl-DL- theanine and 40 mmol/L CoCl2. After 12-h incubation at 50℃ ,22.5mmol/L L-theanine was obtained, the conversion rate against N-Acyl-L-theanine being 90%. CunnighameUa echinulata and the aminoacylase were applied in preparation of L-theanine.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2007年第10期34-38,共5页
China Biotechnology
作者简介
E-mail：jylichn@yahoo．com.cn
