摘要
目的:探讨表没食子儿茶素中没食子酸酯(epigallocatechin-3-ganate,EGCG)对人卵巢癌A2780细胞株及裸鼠移植瘤细胞增殖的抑制、凋亡的诱导作用及相关分子机制。方法:(1)在体外,通过电镜及四甲基偶氮唑盐酶比色法(methyl thiazolyl tetrazolium,MTT)观察不同浓度EGCG在不同时间点作用于A2780细胞后,对其细胞形态及增殖活力的影响。(2)在体内,通过建立A2780细胞裸鼠移植瘤模型,分别比较腹腔注射EGCG低、中、高剂量组和未注射EGCG组之间肿瘤体积及重量的变化;免疫组化及实时荧光定量聚合酶链式反应(real-time polymerase chain reaction,RT-PCR)检测增殖相关基因Ki-67及凋亡相关基因NF-κB(p65)、Bcl-2、Bax蛋白及mRNA的表达情况。结果:体外EGCG诱导A2780细胞的形态学改变;MTT法结果显示,EGCG能显著地抑制A2780细胞的增殖活性,并呈时间-浓度依赖性;体内EGCG注射组与未注射组相比肿瘤体积随用药浓度增加而减小,中、高剂量组与对照组比较差异有统计学意义;免疫组化和RT-PCR结果表明,EGCG能下调Ki-67、NF-κB(p65)、Bcl-2及上调Bax蛋白及mRNA的表达。结论:体外EGCG可有效抑制人卵巢癌A2780细胞的增殖活力;体内EGCG能明显抑制人卵巢癌A2780细胞、裸鼠移植瘤细胞的增殖和诱导凋亡作用,其机制可能与下调Ki-67和NF-κB(p65),促Bax/Bcl-2比值增高有关。
Objective: To investigate the inhibitory effect of epigallocatechin-3-ganate(EGC-G) on the proliferation of human ovarian cancer A2780 cell line and nude mice xenograft tumor cells and the induction of apoptosis and related molecular mechanisms. Methods:(1)To observe the effects of different concentrations of EGCG on A2780 cells at different time points in vitro by using electron microscopy and MTT colorimetry.(2)In vivo, establish A2780 cell trans-plantation in nude mice. Tumor model, comparing changes in tumor volume and weight between low, medium, and high doses of EGCG injected intraperitoneally and non-injected EGCG;immunohistochemical and real-time polymerase chain reaction(RT-PCR) detection of proliferationrelated gene Ki-67 and apoptosis-related gene NF-κB(p65), Bcl-2, Bax protein and mRNA expression.Results: The morphological changes of A2780 cells induced by EGCG in vitro;the results of methyl thiazolyl tetrazolium(MTT)assay showed that EGCG significantly inhibited the proliferation activity of A2780 cells in a time-concentration-dependent manner with statist-ical significance;compared with the EGCG non-injection group, the tumor volume of the injection group decreases with the increasing drug concentration, the difference between the medium and high dose groups and the control group was statistically significant;immunohistochemistry and RT-PCR results showed that EGCG could down-regulate Ki-67 and NF-κB(p65), Bcl-2 and up-regulating the expression of Bax protein and mRNA, the results are statistically significant.Conclusion: EGCG can effectively inhibit the proliferation of human ovarian cancer A2780 cells in vitro;EGCG in vivo can significantly inhibit the proliferation and induce apoptosis of human ovarian cancer A2780 cells and nude mice transplanted tumor cells, and its mechanism may be related to down-regulating Ki-67 and NF-κB(p65), which is related to the increase of Bax/Bcl-2 ratio.
作者
姚金含
李唐娅
张玉泉
YAO Jinhan;LI Tangya;ZHANG Yuquan(Department of Obstetrics and Gynecology,the Affiliated Hospital of Nantong University,Nantong 226001;Department of Obstetrics and Gynecology,Yancheng First People′s Hospital,Jiangsu Provimce)
出处
《南通大学学报（医学版）》
2020年第1期46-50,共5页
Journal of Nantong University(Medical sciences)
作者简介
姚金含,女,汉族,生于1995年11月,辽宁省辽阳市人,硕士,研究方向:妇科肿瘤;通信作者:张玉泉,电话:13706299363,E-mail:jsnt_zhangyuquan@163.com。
