摘要
目的:探讨化学发光免疫分析技术(CLIA)和酶联免疫吸附试验(ELISA)在乙肝病毒血清学检验中的临床应用价值。方法:选取2017年1月至2017年12月收治的102例疑似乙型肝炎患者为研究对象,所有患者均分别采用CLIA方法、ELISA方法进行乙肝病毒血清学检验,采用实时荧光定量PCR检验结果作为参照,比较CLIA、ELISA两种免疫分析技术对诊断乙型肝炎的效果。结果:ELISA方法检测乙肝病毒血清学标志物的灵敏度为93.54%,特异性为95.00%,准确率为94.12%;CLIA方法检测乙肝病毒血清学标志物的灵敏度为95.16%,特异性为97.50%,准确率为96.08%,两种免疫分析技术检测结果比较,差异无统计学意义(P>0.05);CLIA方法、ELISA方法对乙型肝炎的诊断效果结果与实时荧光定量PCR检验结果比较均具有良好的一致性(Kappa分别为0.733、0.709);CLIA方法对HBsAg、HBsAb、HBeAg的阳性检出率分别为93.55%、95.16%、93.55%,明显高于ELISA方法的80.65%、79.03%、80.65%,差异均有统计学意义(P<0.05);表明对乙肝病毒血清学标志物HBsAg、HBsAb、HBeAg的检出效率,CLIA方法优于ELISA方法。CLIA法对HBeAb、HBcAb的阳性检出率分别为90.32%和93.55%,与ELISA法的87.10%、91.94%比较,差异无统计学意义(P>0.05)。结论:CLIA和ELISA在乙肝病毒血清学检验中均具较高的诊断准确率,但CLIA的HBsAg、HBeAg、HBeAb阳性检出率更高。
Objective: To study clinical application values of chemiluminescent immunoassay(CLIA) and enzyme-linked immunosorbent assay(ELISA) in serological test of hepatitis B virus. Methods: 102 patients with suspected hepatitis B infection admitted from January 2017 to December 2017 were selected as the research subjects. The serological test of hepatitis B virus was performed by CLIA method and ELISA method. Then, using the results of real-time fluorescent quantitative PCR as the reference, the effects of CLIA and ELISA in the diagnosis of hepatitis B were compared. Results: The sensitivity, specificity and accuracy of ELISA method in the detection of hepatitis B virus serological markers were 93.54%, 95.00% and 94.12%, separately, while those of the CLIA method were 95.16%, 97.50% and 96.08%, separately, and the differences were not statistically significant(P>0.05). The results of CLIA method and ELISA method in the diagnosis of hepatitis B were in good agreement with those of real-time fluorescent quantitative PCR test(Kappa values were 0.733 and 0.709, respectively). The positive detection rates of HBsAg, HBsAb, and HBeAg by CLIA were 93.55%, 95.16%, and 93.55%, separately, which were significantly higher than those of ELISA method of 80.65%, 79.03%, and 80.65%, and the differences were statistically significant(P<0.05). It showed that the detection efficiencies of CLIA for HBsAg, HBsAb and HBeAg were better than ELISA method. Further, the positive detection rates of HBeAb and HBcAb by CLIA were 90.32% and 93.55% respectively, those by ELISA were 87.10% and 91.94%, and the differences were not statistically significant(P>0.05). Conclusions: CLIA and ELISA both have high diagnostic accuracy in the serological test of hepatitis B virus. However, the positive detection rates of HBsAg, HBsAb, and HBeAg by CLIA are much higher.
作者
左雪灿
丁庆和
李占平
ZUO Xuecan;DING Qinghe;LI Zhanping(Department of Clinical Laboratory of People’s Hospital of Xinzheng,Zhengzhou 451100 Henan,China)
出处
《中国民康医学》
2020年第5期123-125,共3页
Medical Journal of Chinese People’s Health
