摘要
目的:探讨56℃ 30 min灭活血液中的病毒,对光激化学发光法(LiCA)检测输血前传染性指标结果的影响。方法:采集20例患者血液各2管,分别在未灭活和56℃ 30 min灭活后,采用全自动光激化学发光检测仪进行输血前传染性指标[丙型肝炎病毒抗体(HCV–Ab)、人免疫缺陷病毒抗体(HIV–Ab)、梅毒螺旋体抗体(TP–Ab)、乙型肝炎表面抗原(HBsAg)、乙型肝炎表面抗体(HBsAb)、乙型肝炎e抗原(HBeAg)、乙型肝炎e抗体(HBeAb)、乙型肝炎核心抗体(HBcAb)]的检测,比较灭活前后指标的检测结果及发光测定信号值差异。结果:56℃ 30 min灭活前后,所有输血前传染性指标的发光测定信号值比较,差异均无统计学意义(P> 0.05)。所有指标的灭活前后的定性分析符合率均为100%。Pearson相关性分析结果显示,灭活前后,各输血前传染性指标均呈高度的正相关(r均> 0.7,P均<0.01)。结论:血液经56℃ 30 min灭活处理后不会对LiCA检测输血前传染性指标的结果造成影响,该灭活方法可以作为在传染性强的病毒学实验室的标本前处理方法。
Objective To investigate the effect of inactivation of virus in blood at 56 ℃ for 30 min on the results of indicators of pre-transfusion infectivity by light initiated chemilu minescent assay(LiCA). Methods Two tubes of blood from 20 patients were collected, and after inactivation at 56 ℃ for 30 min, the transmissibility indexes [hepatitis C virus antibody(HCV-Ab), human immunodeficiency virus antibody(HIV-Ab), treponema pallidum antibody(TP-Ab), hepatitis B surface antigen(HBsAg), hepatitis B surface antibody(HBsAb), hepatitis B e antigen(HBeAg), hepatitis B e antibody(HBeAb) and hepatitis B core antibody(HBcAb)]were detection, and comparison of the detection results of the indicators before and after inactivation and the difference of the signal value of the luminous assay. Results Before and after inactivation at 56 ℃ for 30 min, there was no statistically significant difference in the signal value of the luminescence measurement of all the infectious indicators before blood transfusion(P > 0.05).The coincidence rate of qualitative analysis before and after inactivation of all indicators was 100 %. Pearson correlation analysis showed that before and after inactivation, the infective indexes before and after transfusion were highly correlated(r > 0.7, P < 0.01).Conclusion The inactivation treatment of blood at 56 ℃ for 30 min will not affect the results of LiCA detection of indicators of pre-transfusion infectivity. This inactivation method can be used as a sample pretreatment method in highly infectious virology laboratories.
作者
彭献香
魏寿忠
PENG Xian-xiang;WEI Shou-zhong(Ningde Hospital Affiliated to Ningde Normal University,Fujian Ningde 352100)
出处
《深圳中西医结合杂志》
2022年第24期15-17,共3页
Shenzhen Journal of Integrated Traditional Chinese and Western Medicine
关键词
56℃30
min灭活病毒
光激化学发光法
输血前传染性指标
Inactivation of virus at 56℃for 30 min
Light initiated chemilu minescent assay
Indicators of pre-transfusion infectivity
作者简介
彭献香,女,主管技师,主要研究方向是临床输血检验;通信作者:魏寿忠,E-mail:weisz2021@163.com,Tel:13559903990。
