摘要
目的:探讨电针刺激百会、印堂穴对慢性不可预见性温和应激(CUMS)抑郁模型大鼠血清炎性因子及单胺类递质的调节作用。方法:将雄性SD大鼠随机分为正常组、模型组、氟西汀组与电针组。除正常组外,其余各组大鼠进行孤养,并接受CUMS刺激方式造模。各组大鼠分别于应激前和应激后进行高架十字迷宫试验并记录数据,观察大鼠的行为学变化。酶联免疫吸附法(ELISA)检测各组大鼠血清白细胞介素-6(IL-6)与肿瘤坏死因子-α(TNF-α)的含量;高效液相色谱法(HPLC)检测血清中去甲肾上腺素(NE)、多巴胺(DA)与5-羟吲哚乙酸(5-HIAA)的含量水平。结果:与模型组比较,高架十字迷宫实验中电针组大鼠进入开臂次数和时间的百分比增加(P<0.01);与模型组比较,电针组IL-6、TNF-α含量显著降低(P<0.01);而相关神经递质含量均有所升高,以NE、DA的改变为最明显(P<0.05),差异具有统计学意义。结论:电针治疗能够改善模型大鼠抑郁症状,抑制血清炎症因子的表达,提高单胺类物质含量,这可能是电针发挥抗抑郁作用的机制之一。
Objective:To study the effects of electro-needling Baihui(GV20)and Yintang(GV29)on the regulation of inflammatory factors and monoamine neurotransmitters in serum of rats with chronic unpredictable mild stress(CUMS)depression.Methods:Sprague-Dawley male rats were randomly divided into the blank control group,the model group,the Fluoxetine group and the electro-acupuncture group.The depression model was established by CUMS plus isolation.The rats in each group were subjected to elevated cross maze test before and after the stimulation,and the behavioral changes of the rats were observed.The contents of cytokines interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)were detected by ELISA.The contents of norepinephrine(NE),dopamine(DA)and 5-hydroxyindoleacetic acid(5-HIAA)were detected by HPLC.Results:Compared to the control group,the numbers of open arm entry(OE)and open arm stay time(OT)were significantly increased(P<0.01)in the electro-acupuncture group.The levels of IL-6 and TNF-αwere significantly decreased in the electro-acupuncture group compared to those in control group(P<0.01).Moreover,the contents of related neurotransmitters were increased;of which the increase of NE and DA were more significant in the electro-acupuncture compared to those in the control group(P<0.05).Conclusion:Electro-acupuncture can improve the depression state of the rats,and inhibit the expressions of inflammatory factors,increase the contents of monoamine neurotransmitters,which may be related to the action mechanism of its anti-depressant effects.
作者
赵俊
田会玲
宋洪涛
王旭
罗彤
艾丽雅
房毓敏
赵江豪
赛音朝克图
李志刚
ZHAO Jun;TIAN Huiling;SONG Hongtao;WANG Xu;LUO Tong;AI Liya;FANG Yumin;ZHAO Jianghao;SAIYIN Chaoketu;LI Zhigang(Beijing University of Chinese Medicine,Beijing 100029,China;Inner Mongolia People′s Hospital,Inner Mongolia 010010,China;Inner Mongolia International Mongolian Hospital,Hohhot 010065,China;Dongfang College of Beijing University of Chinese Medicine,Langfang 065001,China)
出处
《针灸临床杂志》
2019年第12期62-67,共6页
Journal of Clinical Acupuncture and Moxibustion
基金
国家自然科学基金地区科学基金资助项目，编号:81760909
关键词
电针
血清
炎性因子
单胺类递质
抑郁
Electro-acupuncture
Serum
Inflammatory factors
Monoamine neurotransmitters
Depression
作者简介
赵俊(1993-),男,2017级针灸推拿专业硕士研究生;通讯作者:李志刚(1965-),男,主任医师,博士研究生导师,研究方向:针刺干预中枢神经损伤的机理研究。
