摘要
采用溶剂萃取秦巴山区栓皮栎橡子果仁,大孔树脂分离纯化、紫外光谱分析、红外光谱分析、高效液相色谱(High performance liquid chromatography,HPLC)分析栓皮栎橡子果仁多酚的成分组成,DPPH自由基清除率、ABTS^+自由基清除率等、抑菌圈直径研究了其抗氧化活性及抑菌特性。结果表明:栓皮栎橡子果仁粗多酚得率为20.5%,其多酚含量为345 mg/g没食子酸当量,经D4020大孔树脂纯化后多酚得率为34%,多酚含量提高至702 mg/g没食子酸当量;紫外光谱分析、红外光谱分析表明其结构中均具有酚羟基、羰基和芳烃C=C骨架等结构;HPLC分析纯化的橡子果仁多酚至少含有28种物质,其中包括咖啡酸、原儿茶酸、鞣花酸、阿魏酸、槲皮素和山奈酚6种多酚物质,含量分别为24.9、14.6、3.2、2.2、0.5、0.2 mg/g;橡子果仁纯多酚及对照VC清除DPPH自由基的IC50值分别为2.50、3.20μg/mL,清除ABTS+自由基IC50值分别为28.67、28.82μg/mL,ABTS^+自由基清除能力与VC相当,对Fe^3+还原能力与VC差异较小,但对亚铁离子螯合能力远低于EDTA-2Na,抗脂质过氧化能力与VC相当,说明栓皮栎橡子仁多酚具有较强抗氧化能力;栓皮栎橡子仁粗多酚、纯多酚均对阪崎杆菌、沙门氏菌、金黄色葡萄球菌及大肠杆菌具有较强抑制作用,对李斯特菌的抑制作用较弱。研究结果表明栓皮栎橡子果仁多酚含量丰富,其多酚具有较强抗氧化性和广谱抑菌活性,是开发保健品和天然抗菌剂的良好材料。
Quercus variabilis acorn nutlet from Qinba mountainous region in China was extracted by solvent extraction,and the components of polyphenols in the acorn nutlet were investigated by microporous resin separation,UV spectrum,IR spectrum and HPLC analysis.The antioxidative and antibacterial activity of the purified extractives from the acorn nutlet were also evaluated to by scavenging abilities of DPPH free radicals and ABTS+free radicals and inhibition zone.Results showed that the crude polyphenols of acorn nutlet could be extracted up to 20.5%(345 mg/g gallic acid).Purified polyphenols was up to 34%obtained by D4020 microporous resin isolation(702 mg/g gallic acid).By UV-vis spectra and IR spectrum analysis,the purified polyphenols were proved to have phenolic hydroxyl group,carbonyl group and aromatic C=C skeleton structure.HPLC analysis showed that the purified acorn nutlet polyphenols contained at least 28 compounds,including protocatechuic acid,caffeic acid,ferulic acid,ellagic acid,quercetin and kaempferol.The contents were 24.9,14.6,3.2,2.2,0.5,0.2 mg/g.The IC50 values of scavenging DPPH free radicals of acorn nutlet polyphenols and VC were 2.50 and 3.20μg/mL,the IC50 values of scavenging ABTS+free radicals were 28.67,28.82μg/mL.The ability to scavenge DPPH free radicals,ABTS^+free radicals and Fe^3+were similar to that of control VC.The ferrous ions chelating ability was much lower than that of EDTA-2Na.The anti-lipid peroxidation ability could be comparable to VC.The crude extractives and purified extractives all had strong inhibitory effect on Kazakhstan,Salmonella,Staphylococcus aureus and Escherichia coli but weaker inhibitory effect on Listeria.Results revealed that the Quercus variabilis acorn nutlet was rich in polyphenols,the acorn nutlet polyphenols had strong antioxidant activity and broad-spectrum antimicrobial activity,which would be good materials for developing health products and natural antimicrobial agents.
作者
魏园园
刘琪
梁宗瑶
任维维
李珉梦
高鹏程
段旭昌
WEI Yuan-yuan;LIU Qi;LIANG Zong-yao;REN Wei-wei;LI Min-meng;GAO Peng-cheng;DUAN Xu-chang(College of Food Science and Engineering,Northwest A&F University,Yangling 712100,China;College of Natural Resources and Environment,Northwest A&F University,Yangling 712100,China)
出处
《食品工业科技》
CAS
北大核心
2019年第20期42-48,共7页
Science and Technology of Food Industry
基金
国家林业局林业科学技术研究项目(2016-04)
关键词
栓皮栎橡子果仁
多酚提取物
分离纯化
结构
抗氧化活性
抑菌活性
Quercus variabilis acorn nutlet
polyphenol extraction
separation and purification
structure
antioxidative activity
antibacterial activity
作者简介
魏园园(1995-),女,硕士研究生,研究方向:天然产物提取及功能性,E-mail:1094525881@qq.com;通讯作者:段旭昌(1965-),男,博士,副教授,研究方向:天然产物及食品加工新技术,E-mail:duanxc1965@163.com。
