摘要
目的观察高效液相色谱法（HPLC）同时测定白带丸中芍药苷和盐酸小檗碱的含量。方法采用高效液相色谱法。色谱柱：Agilent XDB-C（18）（4.6mm×250mm,5μm）,流动相为乙腈-0.1%甲酸,梯度洗脱,流速：1.0m L/min,柱温30℃,检测波长：245nm。结果芍药苷和盐酸小檗碱线性范围分别为0.18~0.42μg、1.26~2.94μg,芍药苷平均回收率为99.0%,RSD为1.1%（n=6）,盐酸小檗碱平均回收率98.8%,RSD为1.1%（n=6）。结论本法准确可靠、重现性好,为白带丸的质量控制和评价提供了依据。
Objective To establish a method of content determination of Paeoniflorin and Berberine Hydrochloride in Baidai pills by HPLC. Methods HPLC was used to determine the content by Agilent TC -C18 Column（4.6mm×250mm,5μm）, the detection wave deter- mination length at 245nm, the flow rate was 1.0mL/min. Results The calibration curve was linear at the range of 0.18 - 0.42μg for Paeon- iflorin and 1.26 -2.94μg for Berberine Hydrocbloride. The average recovery of Paeoniflorin was 99.0% and RSD was 1.1% （ n =6）. The average recovery of Berberine Hydrochloride was 98.8 % and RSD was 1.1% （ n = 6）. Conclusion This method was accurate and proper with good reproducibility. It can be used for quality control of Baidai pills.
出处
《湖北中医药大学学报》
2016年第3期57-59,共3页
Journal of Hubei University of Chinese Medicine
作者简介
黄渭（1986-），男，武汉市中心医院药剂师，研究方向：主要从事中药及其制剂的质量标准研究。
通讯作者：张丽君（1975-），女，湖北中医药大学实验师，硕士研究生，研究方向：主要从事生物化学及中药药理方面的研究，E-mail：316923124@qq.com
