摘要
目的:探讨推拿对失神经骨骼肌萎缩大鼠的治疗作用及其机制。方法:48只雄性SD大鼠随机分为模型组(n=24)和推拿组(n=24),通过切断右侧胫神经制备腓肠肌萎缩大鼠模型。术后第2日开始给推拿组大鼠手术侧腓肠肌给予手法干预,模型组不予干预。两组分别在0d、7d、14d、21d四个时间点各处死6只大鼠,取大鼠双侧腓肠肌,称重后计算各组大鼠腓肠肌湿重比;HE染色测定肌纤维截面积和直径,实时荧光定量PCR检测腓肠肌中miR-23a、Akt、MuRF1、MAFbx基因相对表达量。结果:与0d比较,模型组和推拿组大鼠腓肠肌湿重比、肌纤维截面积和直径呈现进行性下降的趋势,其中7d、14d、21d推拿组腓肠肌湿重比、肌纤维截面积和直径均显著高于模型组(P<0.05,P<0.01);与0d比较,模型组和推拿组MuRF1、MAFbx、AktmRNA表达均呈现先升后降的趋势,其中7d、21d推拿组MuRF1mRNA表达均显著低于模型组(P<0.05,P<0.01),7d、14d、21d推拿组MAFbxmRNA表达均显著低于模型组(P<0.01,P<0.05,P<0.01),7d、14d、21d推拿组AktmRNA表达均显著高于模型组(P<0.05,P<0.01);与0d比较,模型组和推拿组21d时miR-23amRNA表达升高,推拿组miR-23amRNA表达显著高于模型组(P<0.05)。结论:推拿能延缓失神经骨骼肌的萎缩,其机制可能与上调miR-23a、Akt基因的表达,下调MuRF1、MAFbx基因的表达,使蛋白降解速度受到抑制,从而减轻骨骼肌蛋白的降解程度有关。
Objective: To investigate the therapeutic effects of massage on denervated skeletal muscle atrophy in rats and its mechanism. Methods: Forty-eight male SD rats were randomly divided into model group( n = 24) and massage group( n = 24). Gastrocnemius muscle atrophy model was established by transecting the right tibial nerve of rat. On the second day after operation,the gastrocnemius muscle of the rats in the massage group was given manual intervention and the model group was not intervened. Six rats were sacrificed at the four time points of 0 d,7 d,14 d and 21 d. The gastrocnemius of the rats were obtained and measured the wet mass ratio after weighing. Cross-sectional area and diameter of the muscle fiber were measured after HE staining. The relative expressions of miR-23 a,Akt,MuRF1 and MAFbx mRNA were tested with q PCR. Results: Compared with 0 d,the wet weight ratio,cross-sectional area and diameter of gastrocnemius muscle showed a progressive decline in the model group and massage group. The wet weight ratio,cross-sectional area and diameter of gastrocnemius muscle in the massage group were higher than those in the model group on 7 d,14 d and 21 d( P<0.05,P<0.01). Compared with 0 d,the expressions of MuRF1,MAFbx and Akt mRNA were increased first and then were decreased in the model group and massage group. The expression of MuRF1 mRNA in massage group was lower than that in model group on 7 d and 21 d( P<0.05,P<0.01). The expression of MAFbx mRNA in massage group was lower than that in model group on7 d,14 d and 21 d( P<0.01,P<0.05,P<0.01). The expression of Akt mRNA in massage group was higher than that in model group on 7 d,14 d and 21 d( P<0.05,P<0.01). Compared with 0 d,the expression of miR-23 a mRNA was increased in the model group and massage group on 21 d,and the expression of miR-23 a mRNA in massage group was higher than that in model group( P< 0.05).Conclusion: Massage can delay the atrophy of denervated skeletal muscle. The mechanism may be related to up-regulation of the expression of miR-23 a and Akt mRNA,down-regulation of the expressions of MuRF1 and MAFbx mRNA,inhibition of protein degradation rate,and reduction of skeletal muscle protein degradation.
作者
万小凤
唐成林
赵丹丹
安荟羽
马翔
谯童茜
WAN Xiao-feng;TANG Cheng-lin;ZHAO Dan-dan;AN Hui-yu;MA Xiang;QIAO Tong-xi(College of Traditional Chinese Medicine,Chongqing Medical University,Chongqing 400016;Chongqing Key Laboratory ofTraditional Chinese Medicine for Prevention and Cure of Metabolic Diseases,Chongqing 400016,China)
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2019年第3期223-227,267,I0001,共7页
Chinese Journal of Applied Physiology
基金
国家自然科学基金项目(81273870)
重庆市卫生和计划生育委员会、重庆市科学技术委员会联合资助中医药重点科研项目(ZY201801007)
关键词
失神经
骨骼肌萎缩
推拿
蛋白降解
大鼠
denervation
muscle atrophy
massage
protein degradation
rats
作者简介
通讯作者:唐成林,Tel:13452083746;E-mail:cytcl966@163.com.
