摘要
目的:探讨miR-193a-5p靶向CDK14并调控卵巢癌细胞OVAC的增殖和上皮间充质转变(EMT)的作用。方法:通过Target Scan Human分析miR-193a-5p与CDK14的匹配情况,通过荧光素酶报告系统检测miR-193a-5p靶向CDK14情况;在miR-193a-5p mimics过表达或者miR-193a-5p inhibitor基因沉默miR-193a-5p的情况下,采用免疫印迹检测CDK14,EMT相关蛋白质E-cadherin、vimentin、fibronectin和N-cadherin的表达量,采用CCK-8检测卵巢癌细胞OVAC增殖情况,MMT检测卵巢癌细胞OVAC的细胞活力。结果:miR-193a-5p靶向CDK14的3‘UTR;过表达miR-193a-5后,CDK14的表达下降,EMT相关蛋白质E-cadherin的表达上升,vimentin、fibronectin和N-cadherin的表达下降,卵巢癌细胞OVAC的增殖和细胞活力均增加;同时,基因沉默miR-193a-5p后,CDK14的表达上升,EMT相关蛋白质E-cadherin的表达下降,vimentin、fibronectin和N-cadherin的表达量上升,卵巢癌细胞OVAC的增殖和细胞活力均减少。结论:miR-193a-5p通过靶向CDK14的3‘UTR降低卵巢癌细胞OVAC的增殖、细胞活力和EMT。
Objective:To investigate whether miR-193a-5p targets CDK14 and regulates the proliferation and epithelial-mesenchymal transition(EMT)of ovarian cancer cell line OVAC.Methods:Target Scan Human was used to analyze the match between miR-193a-5p and CDK14,and then miRNA assay was used to detect whether miR-193a-5p targeting CDK14;miR-193a-5p mimics overexpression or miR-193a-5p inhibitor knockdown in the case of low miR-193a-5p,the expression levels of CDK14,EMT-related proteins E-cadherin,vimentin,fibronectin and N-cadherin were detected by immunoblotting,and the proliferation of ovarian cancer cells OVAC was detected by CCK-8,and the cell viability of cancer cell OVAC was detected by MMT.Results:miR-193a-5p targeted the 3’UTR of CDK14;after overexpression of miR-193 a-5,the expression of CDK14 was decreased,the expression of EMT-related protein E-cadherin was increased,and the expressions of vimentin,fibronectin and N-cadherin were decreased.The proliferation and cell viability of ovarian cancer cell line OVAC were increased.Meanwhile,after knocking down miR-193a-5p,the expression of CDK14 was increased,and the expression of EMT-related protein E-cadherin was decreased,while the expression levels of vimentin,fibronectin and N-cadherin were increased,and the proliferation and cell viability of ovarian cancer cell line OVAC were decreased.Conclusion:miR-193a-5p reduces the proliferation,cell viability and EMT of ovarian cancer cell line OVAC by targeting the 3’UTR of CDK14.
作者
杨尊敬
杜先玲
YANG Zun-jing;DU Xian-ling(Department of Oncology,Enshi Central Hospital,Enshi 445000,China)
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2020年第2期176-180,共5页
Chinese Journal of Applied Physiology
作者简介
通讯作者:杜先玲,E-mail:cvhbofh@sina.com
