摘要
目的:考察不同负荷运动训练对小鼠心肌凋亡相关miR-1,miR-21和靶蛋白的影响,探讨运动干预心肌凋亡的可能机制。方法:选取21只C57BL/6小鼠,随机分为3组(n=7):安静组(SE组)、训练1组(ET1组)、训练2组(ET2)。SE组不进行训练,ET1组完成8周递增负荷游泳训练,5天/周,1次/天,第1周30 min/count,每周增加10 min,第7、8周时间维持在90 min;ET2组在ET1组方案基础上增加负荷,前5周与ET1相同,后3周每天训练2次。TUNEL检测考察心肌凋亡水平,Western blot和RT-PCR分别测定蛋白和miRs的变化。结果:ET1组游泳训练对小鼠心肌凋亡影响不明显,miR-1表达无显著变化,但其靶蛋白Bcl-2表达显著增高(P<0.01),miR-21及其靶蛋白PDCD4表达均无显著变化。ET2组游泳训练显著降低心肌凋亡水平及miR-1表达(P<0.01)、提高Bcl-2表达(P<0.05);同时显著提高miR-21表达(P<0.05),但对PDCD4表达无明显影响。结论:ET1组训练对心肌凋亡干预不明显,ET2组运动训练可降低心肌凋亡水平,miR-1及靶蛋白Bcl-2变化可能是机制之一,PDCD4对运动训练不敏感,miR-21可能与其它靶蛋白参与运动干预心肌凋亡的分子机制。
Objective： To detect the levels of miR-1,miR-21 and their targeted proteins in hearts of mice after different exercise training,and discuss potential molecular mechanism. Methods： Male C57 BL/6 mice were randomly divided to 3 groups： sedentary（ SE）,exercise training 1（ ET1） and exercise training 2（ ET2）. SE did not do any exercise; ET1 undertook swimming training for 8 weeks,once a day,5 days/week. Swimming 30 min in the 1 stweek,and the duration was increased 10 min per week to 90 min and maintained in the 7 thand 8 thweek. ET2 performed the same work as ET1 and switched to twice a day by the end of the 5 th week.TUNEL assay was applied to test myocardial apoptosis. Western blot and RT-PCR were used to detect proteins and miRs levels respectively. Results： Compared with SE,in ET1,myocardial apoptosis and miR-1 level did not change,but its targeted protein Bcl-2 increased significantly（ P〈0.01）. miR-21 and its targeted protein PDCD4 did not change significantly. In ET2,myocardial apoptosis and miR-1 level were decreased significantly（ P〈0.05）. Bcl-2 was increased significantly（ P〈0.01）. miR-21 also increased significantly（ P〈0.05）,but PDCD4 did not decrease significantly. Conclusion： Exercise training in ET2 other than ET1 could down-regulate myocardial apoptosis. Alterations of miR-1 and Bcl-2 may be responsible for this cardioprotection. PDCD4 is not sensitive to exercise training,it is likely that miR-21 and other targeted proteins participate in exercise-regulative apoptosis.
作者
赵永才
付近梅
高炳宏
ZHAO Yong-cai;FU Jin-mei;GAO Bing-hong(Shanghai University of Sport, Shanghai 200438;Institute for Sports Science of Jiangxi Province, Nanchang 350006, China)
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2018年第1期93-96,共4页
Chinese Journal of Applied Physiology
基金
上海市科学技术委员会重点攻关项目(15dz1208001)
上海市人类运动能力开发与保障重点实验室(17DZ2273100)
关键词
运动
心肌
微小RNA
凋亡
小鼠
exercise
cardiomyocyte
microRNAs
apoptosis
mice
作者简介
通讯作者：高炳宏，Tel：021—51253167；E-mail：gaobinghong@126．com
