摘要
为研究杨梅酮的抗氧化和抗膀胱癌活性,进行了抗氧化试验和环境扫描电子显微镜(ESEM)观察.结果表明,杨梅酮能有效抑制AAPH诱导的红细胞溶血,且效果呈浓度依赖性,80μmol/L杨梅酮的溶血抑制率达到(93.67±0.01)%.研究表明,杨梅酮可通过增强细胞内SOD、GPx的酶活来调控ROS水平,减少MDA产生,避免脂质过氧化.抗肿瘤活性研究发现,杨梅酮对膀胱癌细胞EJ和T24以及正常膀胱细胞SV-HUC-1的抑制效果也有剂量相关性,IC_(50)分别为(39.8±1.0)、(49.0±0.9)、(75.9±1.8)μmol/L.进一步细胞吸收量分析表明,可能是EJ对杨梅酮有更强的细胞吸收导致了杨梅酮对EJ的IC_(50)比T24更低(更敏感的细胞毒性).采用流式细胞术分析杨梅酮对EJ细胞周期DNA分布的影响,发现杨梅酮主要通过诱导EJ细胞产生凋亡及S期阻滞抑制细胞增殖.
Antioxidant assay and ESEM observation were carried out to investigate the antioxidant and anti-bladder cancer activities of myricetin. The results show that myricetin can effectively inhibit the AAPH-induced hemolysis of erythrocytes in a dose-dependent manner. The hemolysis inhibition rate of myricetin increases to(93.67±0.01)%at a concentration of 80 μmol/L. A further experiment confirms that SOD and GPx antioxidant enzyme activities are enhanced to control ROS and MDA generation when erythrocytes are preconditioned by myricetin. Moreover, MTT tests indicate that myricetin could suppress the EJ and T24 bladder cancer cells proliferation in a dose-related manner with the half-maximal inhibitory concentration(IC50) of(39.8±1.0) μmol/L and(49.0±0.9) μmol/L, as well as a lower cytotoxicity on normal bladder cell SV-HUC-1 with the IC50 of(75.9±1.8) μmol/L. In addition, myricetin exhibits higher antitumor activity in EJ cell than that in T24 cells due to higher intracellular uptake amount of myricetin in EJ cells. Further flow cytometric studies of EJ cell cycle distribution show that myricetin inhibits the proliferation of EJ cancer cells mainly through induction of apoptosis and S cycle arrest.
作者
苏健裕
胡菡
吴萍
孟晓风
徐振波
郑华德
SU Jianyu;HU Han;WU Ping;MENG Xiaofeng;XU Zhenbo;ZHENG Huade(School of Food Science and Engineering,South China University of Technology,Guangzhou 510640,Guangdong,China;School of Materials Science and Engineering,South China University of Technology,Guangzhou 510640,Guangdong,China;South China Institute of Collaborative Innovation,Dongguan 523000,Guangdong,China)
出处
《华南理工大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
2019年第3期101-108,152,共9页
Journal of South China University of Technology(Natural Science Edition)
基金
广东省科技计划项目(2016A040402032
2016A010105020
2016A040402016)
广东省扬帆计划项目(201312H05)
广州市科技计划项目(201707010129)
华南理工大学中央高校基本科研业务费专项资金项目(2017ZD084)
揭阳市科技计划项目(2017xm020)~~
关键词
类黄酮
抗氧化活性
抗肿瘤
flavonoids
antioxidant activity
antitumor
作者简介
苏健裕(1979-),男,博士,副研究员,主要从事食品生物技术研究,E-mail:jysu@scut.edu.cn;通信作者:郑华德(1978-),男,博士,副研究员,主要从事医用生物材料研究.E-mail:hdzheng@scut.edu.cn.
