摘要
目的:研究复方胃炎胶囊对胃炎模型大鼠胃液游离酸度、胃蛋白酶活性及胃动力障碍模型小鼠胃排空的影响,为其开发利用提供参考。方法:将72只大鼠随机分为正常组(n=12)和造模组(n=60),造模组大鼠采用60%乙醇联合熊去氧胆酸制备大鼠胃炎模型。造模成功后,将55只大鼠随机分为模型组、雷尼替丁组(40 mg/kg)和复方胃炎胶囊高、中、低剂量组(相当于原生药16、8、4 g/kg),每组11只。各给药组大鼠每天灌胃给药1次,正常组和模型组大鼠灌胃等体积0.5%羧甲基纤维素钠(0.5%CMC)溶液,连续给药28 d。给药结束后,检测各组大鼠胃液游离酸度和胃蛋白酶活性,苏木精-伊红染色后观察各组大鼠胃组织病理学变化并进行炎症评分。将60只小鼠随机分为正常组、模型组、多潘立酮组(50 mg/kg)和复方胃炎胶囊高、中、低剂量组(相当于原生药20、10、5 g/kg),每组10只。各给药组小鼠灌胃相应药液,正常组和模型组小鼠灌胃等体积0.5%CMC溶液。末次给药50 min后,除正常组外,其余各组小鼠均腹腔注射阿托品3 mg/kg制备胃动力障碍模型。造模20 min后,测定小鼠胃内残留率。结果:与正常组比较,模型组大鼠胃液游离酸度和胃蛋白酶活性显著降低(P<0.05),胃黏膜上皮细胞排列紊乱、有大量炎性细胞浸润,炎症评分显著升高(P<0.05);模型组小鼠胃内残留率显著升高(P<0.05)。与模型组比较,雷尼替丁组和复方胃炎胶囊高剂量组大鼠胃液游离酸度和胃蛋白酶活性显著升高(P<0.05),胃组织病理学损伤明显减轻,炎症评分显著降低(P<0.05);多潘立酮片组和复方胃炎胶囊高剂量组小鼠胃内残留率显著降低(P<0.05)。结论:复方胃炎胶囊可提高胃炎模型大鼠的胃液游离酸度和胃蛋白酶活性,改善胃组织病理变化,并可促进胃动力障碍模型小鼠的胃排空。
OBJECTIVE:To study the effects of Compound gastritis capsule on free acidity and protease activity of gastric juice in gastritis model rats and gastric emptying in gastric motility disorder model mice,and to provide reference for its development and utilization.METHODS:Totally 72 rats were randomly divided into normal group(n=12)and model group(n=60).Model group was given 60%ethanol combined with ursodeoxycholic acid to induce gastritis model of rats.After modeling,55 rats were randomly divided into model group,ranitidine group(40 mg/kg)and Compound gastritis high-dose,medium-dose and low-dose groups(equivalent to 16,8,4 g/kg crude drug),with 11 rats in each group.Rats of administration groups were given relevant drug intragastrically once a day.Normal group and model group were given constant volume of 0.5%carboxymethylcellulose sodium(0.5%CMC)solution for consecutive 28 d.After medication,free acidity and protease activity of gastric juice in gastritis model rats were determined;after HE staining,the pathological changes of gastric tissue were observed and the inflammatory score was observed.Totally 60 mice were randomly divided into normal group,model group,domperidone group(50 mg/kg),Compound gastritis capsule high-dose,medium-dose and low-dose groups(equivalent to 20,10,5 g/kg crude drug),with 10 mice in each group.Administration groups were given relevant medicine liquid intragastrically,and mice of normal group and model group were given constant volume of 0.5%CMC solution intragastrically.50 min after last medication,except for normal group,other groups were given atropine 3 mg/kg intraperitoneally to induce gastric motility disorder model.The residual rate of drug in the stomach of mice was determined 20 min after modeling.RESULTS:Compared with normol group,the free acidity and protease activity of gastric juice in rats in model group were increased significantly(P<0.05),and the epithelial cells in the gastric mucosa were in disorder,a large number of inflammatory cells were infiltrated,the score of inflammation was increased significantly(P<0.05);the residual rate of drug in the stomach of mice in model group was increased significantly(P<0.05).Compared with model group,the free acidity and protease activity of gastric juice in rats in in ranitidine group and Compound gastritis capsule high-dose group was significantly decreased(P<0.05),the histopathological injury of stomach was obviously reduced and the score of inflammation was significantly decreased(P<0.05);the residual rate in the stomach of mice in domperidone group and Compound gastritis capsule high-dose group was significantly decreased(P<0.05).CONCLUSIONS:Compound gastritis capsule can improve the free acidity and protease activity of gastric juice in gastritis model rat and improve the pathological changes of gastric tissue,and can promote the gastric emptying of the gastric motility disorder model mice.
作者
郑姣妮
张颖
刘玲
ZHENG Jiaoni;ZHANG Ying;LIU Ling(Dept.of Pharmacy,Chongqing the Forth Hospital,Chongqing 400014,China)
出处
《中国药房》
CAS
北大核心
2018年第17期2360-2364,共5页
China Pharmacy
基金
重庆市卫生局中医药科研计划项目(No.2012-2-31)
关键词
复方胃炎胶囊
胃炎模型
胃液酸度
胃蛋白酶活性
胃动力障碍模型
胃排空
小鼠
大鼠
Compound gastritis capsule
Gastritis model
Acidity of gastric juice
Protease activity of gastric juice
Gastric motility disorder model
Gastric emptying
Mice
Rat
作者简介
郑姣妮:主管药师,硕士。研究方向:临床药学、药物治疗学。电话:023-63692203。E-mail:78625529@qq.com;通信作者:刘玲:主任药师。研究方向:医院药学、临床药学。电话:023-63692201。E-mail:494038962@qq.com
