摘要
目的初步探讨祛风解痉汤对咳嗽变异性哮喘(CVA)豚鼠气道炎性反应的影响作用。方法 40只SPF级雌性豚鼠按随机数字表法随机分为正常对照组、模型组、阳性对照组、中药高剂量组、中药低剂量组,每组8只。采用鸡清白蛋白(OVA)、氢氧化铝腹腔注射致敏并OVA激发法复制CVA豚鼠模型。阳性对照组、中药高剂量组、中药低剂量组于雾化当天开始分别以醋酸泼尼松3 mg/kg、祛风解痉汤32.88 g/kg、祛风解痉汤8.22 g/kg灌胃。正常对照组及模型组给予等量的生理盐水灌胃。检测各组豚鼠咳嗽敏感性(CRS)、支气管肺泡灌洗液(BALF)中嗜酸粒细胞(EOS)百分率及血清中白介素-4(IL-4)、IL-12、IL-13、肿瘤坏死因子β(TNF-β)、γ干扰素(IFN-γ)水平。结果模型组CRS、EOS百分率及血清IL-4、IL-12、IL-13、TNF-β、INF-γ表达水平明显高于正常对照组,差异有统计学意义(P<0.05)。阳性对照组、中药高剂量组、中药低剂量组豚鼠的CRS、EOS百分率明显低于模型组,阳性对照组及中药高剂量组血清IL-4、IL-12、IL-13、TNF-β、INF-γ表达水平明显低于模型组,差异均有统计学意义(P<0.05)。(3)中药高剂量组豚鼠CRS、EOS百分率及血清TNF-β、INF-γ表达水平明显低于中药低剂量组,差异有统计学意义(P<0.05);结论祛风解痉汤能明显抑制CVA豚鼠CRS,降低CVA豚鼠BALF中EOS百分率,亦能一定程度减轻血清炎性因子表达,相关机制尚需优化处方配伍深入探讨。
Objective To preliminarily investigate the efficacy of Qufeng Jiejing Decoction on airway inflammation reaction in guinea pigs with cough variant asthma(CVA).Methods Forty SPF female guinea pigs were divided into five groups with eight Digs in each group by random number table,including control group,model group,positive drug group,low-dose Qufeng Jiejing Decoction group and high-dose Qufeng Jiejing Decoction group.The OVA-induced method,by injecting OVA and aluminum hydroxide(ALP)intraperitoneally,was applied to make guinea pig CVA model.On the date of nebulization,the positive drug group,high-dose and low-dose Qufeng Jiejing Decoction groups were respectively treated with prednisone acetate at 3 mg/kg,Qufeng Jiejing Decoction at doses of 2.88 g/kg and 8.22 g/kg by gavage.The control group and model group were treated with normal saline at the same time.The cough response sensitivity(CRS),eosinophil(EOS)percentage in bronchoalveolar lavage fluid(BLAF),as well as levels of serum interleukin-4(IL-4),IL-12,IL-13,tumor necrosis factorβ(TNF-β)and interferon-γ(IFN-γ)of each group were detected.Results Compared with the control group,the CRS,EOS percentage,and the expression levels of IL-4,IL-12,IL-13,TNF-βand INF-γin serum of the model group were increased significantly(P<0.05).Compared with the model group,the CRS and EOS percentage were significantly decreased in the positive drug group,high-dose and low-dose Qufeng Jiejing Decoction group,while the levels of serum IL-4,IL-12,IL-13,TNF-βand INF-γwere significantly reduced(P<0.05).Compared with the low-dose Qufeng Jiejing Decoction group,the CRS,EOS percentage,and the levels of TNF-βand INF-γin high-dose Qufeng Jiejing Decoction group were reduced significantly(P<0.05).Conclusion Qufeng Jiejing Decoction can evidently inhibit the CRS and the EOS percentage of CVA guinea pigs,and also reduce the expression of inflammatory cytokines in their serum to some extent.However,the relevant mechanism is worth investigating and needs further exploration.
作者
宋苹
张溪
张瞳
张忠德
SONG Ping;ZHANG Xi;ZHANG Tong;ZHANG Zhongde(Lingnan Zhen Shi Miscellaneous Disease School Outpatient,Guangdong Province Traditional Chinese Medical Hospital,Guangdong Province,Guangzhou 510120,China)
出处
《中国医药导报》
CAS
2018年第22期14-17,共4页
China Medical Herald
基金
国家中医临床研究基地业务建设科研专项课题(JDZX2015200)
作者简介
[通讯作者]张忠德(1964-),男,汉族,硕士,广东省中医院副院长,主任医师,教授,博士研究生导师;研究方向:中医药防治呼吸系统疾病的临床与基础研究。
