摘要
为研究野生大豆种皮障碍休眠解除及萌发的有效方法,采取98%浓硫酸、过氧化氢、80℃热水浴、刻伤方法进行处理,研究其对野生大豆种子萌发、生物量、吸水种子重量变化规律、保护酶、内肽酶等指标的影响。结果表明:刻伤处理大豆种子发芽势、发芽率及发芽指数均最高,显著优于其它处理,98%浓硫酸10 min次之。胚轴长刻伤处理下值最大,胚根长及鲜重以98%浓硫酸10 min处理下值最大,发霉率以80℃热水浴处理下值最低,硬实率以刻伤处理下值最低。处理和对照的吸水后种子重量随时间的后延均呈现出明显的'S'曲线规律,其中,98%浓硫酸10 min、过氧化氢15 min、80℃热水浴5 min、刻伤和对照处理吸水后种子重量从开始吸水期进入滞缓期的转折点分别出现在9,12,9,6和15 h,从滞缓期进入重新大量吸水期的转折点分别出现在24,27,27,24和30 h,以刻伤和98%浓硫酸处理下种子萌动最早。98%浓硫酸10 min和80℃热水浴处理下,SOD、POD活性较大,而刻伤和98%浓硫酸10 min处理下,内肽酶活性较大,可溶性蛋白质含量较高。综合而言,刻伤处理最有利于野生大豆种子种皮障碍休眠的解除,其次是98%浓硫酸处理10 min。
In order to promote seed germination and break dormancy of wild soybean,98%concentrated sulfuric acid,hydrogen peroxide,hot water bath at 80℃and wounding were used to study their effects on seed germination index,biomass and water absorption law of wild soybean.The results showed that the germination potential,germination rate and germination index of soybean seeds treated with scuffing were the highest,which was significantly superior to those of other treatments,followed by 98%concentrated sulfuric acid for 10 min.The lowest values of hypocotyl length were obtained by scuffing treatment,radicle length and fresh weight by 98%concentrated sulfuric acid for 10 min,mildew rate by water bath at 80℃,and hardness rate by scuffing treatment.The’S’curve of seed weight after treatment and control was obvious.The turning point of seed weight from the initial absorption period to the delay appeared in 9 h after 98%concentrated sulphuric acid treated for 10 min,12 h after hydrogen peroxide treated for 15 min,9 h after 80℃water bath for 5 min,6 h after scratched,15 h in contrast.The turning point from the delay to the period of re-absorption of large amounts of water appeared in the 24,27,27,24 and 30 h respectively in the five treatments.The seed sprouting earliest under the treatment of scuffing and 98%concentrated sulfuric acid treated.The activities of SOD and POD were higher under the treatment of 98%concentrated sulfuric acid for 10 min and 80℃water bath,while the activities of endopeptidase and soluble protein were higher under the treatment of burn and 98%concentrated sulfuric acid for 10 min.Generally,the treatment of scuffing is the best way to relieve seed coat obstacle dormancy of wild soybean,followed by 98%concentrated sulfuric acid for 10 min.
作者
王付娟
刘书含
李淑梅
周强
雷振山
WANG Fu-juan;LIU Shu-han;LI Shu-mei;ZHOU Qiang;LEI Zhen-shan(Xinyang College of Agriculture and Forestry,Xinyang 464000,China)
出处
《大豆科学》
CAS
CSCD
北大核心
2019年第5期733-739,共7页
Soybean Science
基金
河南省科技攻关(172102110126)
关键词
野生大豆
萌发
生物量
吸水规律
蛋白质代谢
Wild soybean
Germination
Biomass
Water absorption law
Protein metabolism
作者简介
第一作者简介:王付娟(1981-),女,硕士,讲师,主要从事作物遗传育种学教学与研究。E-mail:wfj703@126.com;通讯作者:雷振山(1974-),男,硕士,副教授,主要从事作物学研究。E-mail:leizs2003@xyafu.edu.cn。
