摘要
目的:探讨不同浓度丹参酮ⅡA对软骨细胞Ⅱ型胶原及Wnt/β-catenin信号通路的影响。方法:提取新生SD大鼠原代软骨细胞,并通过Ⅱ型胶原蛋白免疫荧光进行鉴定,取P1代细胞进行实验,共设5组,其中1组设为空白对照组,另外4组分别用6.25μmol/L,12.5μmol/L,25μmol/L及50μmol/L浓度丹参酮ⅡA对其进行干预,24h后通过蛋白印迹分析法(Western Blot)检测软骨细胞Ⅱ型胶原及β-catenin蛋白的表达情况。结果:随着丹参酮ⅡA浓度的增加,Ⅱ型胶原蛋白表达增加,12.5μmol/L,25μmol/L及50μmol/L丹参酮ⅡA组Ⅱ型胶原蛋白量均较对照组增高(P<0.05),且Ⅱ型胶原蛋白量与丹参酮ⅡA浓度呈正相关(r=0.949,P<0.001),各丹参酮ⅡA组β-catenin蛋白量均较对照组降低(P<0.05),且β-catenin蛋白量与丹参酮ⅡA浓度呈负相关(r=-0.949,P<0.001)。结论:丹参酮ⅡA可抑制Wnt/β-catenin信号通路,促进软骨细胞Ⅱ型胶原表达,延缓软骨细胞退变。
Objective：To investigate the effect of Tanshinone ⅡA（TAN ⅡA）on collagen Ⅱ and Wnt/β-catenin signal pathway of rat chondrocytes in vitro.Methods：SD rat articular chondrocytes were isolated,and immunofluorescence was performed to identify the cells.A total of 5 groups were set up,1 of which was a blank control group,and the other 4 groups were treated with different concentrations of TAN ⅡA（6.25μmol/L,12.5μmol/L,25μmol/L and 50μmol/L）.The expression ofβ-catenin and collagen Ⅱ were examined by Western-Blot after 24 h.Results：The groups of12.5μmol/L,25μmol/L,50μmol/L concentration of TAN ⅡA expressed higher collagenⅡthan the control group（P〈0.05）.The expression of collagenⅡ was increased as the concentration of TAN ⅡA had a rising trend（r=0.949,P〈0.001）.All the groups treated with TAN ⅡA expressed lowerβ-catenin than the control group（P〈0.05）.The expression ofβ-catenin was decreased as the concentration of TAN ⅡA had a rising trend（r=-0.949,P〈0.001）.Conclusion：TAN ⅡA can inhibit the Wnt/β-catenin signaling pathway,promote the expression of type Ⅱ collagen in chondrocytes,and delay the degeneration of chondrocytes.
作者
宋奕
朱寅
丁道芳
SONG Yi;ZHU Yin;DING Daofang(Suzhou Hospital of Integrated Chinese and Western,Suzhou 215000,Jiangsu China;Shi＇s Center of Orthopedics and Traumatology,Shuguang Hospital Affiliated to Shanghai University of TCM,Shanghai 201203,China)
出处
《中国中医骨伤科杂志》
CAS
2018年第9期1-4,共4页
Chinese Journal of Traditional Medical Traumatology & Orthopedics
基金
苏州市科技局指导性项目(SYSD2016051,SYSD2016050)
作者简介
通信作者:朱寅,E-mail:395344510@qq.com
