摘要
目的通过观察丹参加玻璃酸钠关节内注射,探讨其对兔膝关节软骨的保护效应与机制。方法通过切断膝关节前交叉韧带和内侧副韧带造成膝OA模型,将80只日本大耳白兔随机分为A组、B组、C组、D组。给药4、8周后处死实验兔,行软骨细胞凋亡率检测。结果模后第4周、8周,B、C、D三组软骨细胞凋亡率著低于A组(P<0.01),A、B、C、D组组间软骨细胞凋亡率比较,有显著性差异(P<0.01);B、C、D组组间比较,差异无统计学意义(P>0.05),但D组凋亡率明显小于B、C组。结论丹参加玻璃酸钠关节内注射,能明显降低兔膝关节软骨的细胞凋亡率,减缓膝关节软骨的退变,对软骨起保护效应。
Objective To observe the effects and mechanism of Radix Salviae Miltiorrhizae and Sodium Hyaluronate on cartilage of osteoarthritis in rabbit knee joints. Methods Knee osteoarthritis model was created by cutting off the anterior cruciate ligament and medial collateral ligament in the knee joint. 80 adult Japanese white rabbits were divided randomly into 4 groups： Group A, Group B, Group C, Group D. After drug treatment for 4 and 8 weeks, the rabbits were killed and the ratio of chondrocyte apopto- sis was detected. Results After 4 and 8weeks, the ratio of chondrocyte apoptosis in group B, C or D was smaller than that in group A（P 〈0.01）. There had significant difference among A,B,C,D groups （P 〈0.01） , but there had no significant differ- ence among B, C or D group （P 〉 0.05 ）. The ratio of chondrocyte apoptosis in group D was smaller than that in group B or C （P 〉 0.05 ）. Conclusion Radix Salviae Miltiorrhizae and the Sodium Hyaluronat can markedly inhibit the rate of chondrocytes apop- tosis in osteoarthritis, and has the effect of protecting chondrocyte and delay the occurrence.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2012年第10期2452-2453,共2页
Lishizhen Medicine and Materia Medica Research
基金
广西壮族自治区自然科学基金(No.0542104)
关键词
骨关节炎
丹参
玻璃酸钠
细胞凋亡
效应
机制
兔
Osteoarthtitis
Radix Salviae Miltiorrhizae （ RSM ）
Sodium Hyaluronate （ SH ）
Apoptosis
Effects
Mechanism
Knee joint/rabbit
作者简介
袁长深（1978-），男（汉族），广西桂林人，现任广西中医药大学第一附属医院主治医师，硕士学位，主要从事骨与关节疾病的防治研究工作．
段戡（1964-），男（汉族），湖南冷水江人，现任广西中医药大学第一附属医院主任医师，博士学位，主要从事骨与关节疾病防治工作．
