摘要
以山西介休金山坡煤矿采煤塌陷土壤为对象,通过接种幼套球囊霉（Glomus etunicatum,G.e）、根内球囊霉（G.intraradice,G.i）和摩西球囊霉（G.mosseae,G.m）3种丛枝菌根（Arbuscular mycorrhiza,AM）真菌,研究不同菌种对采煤塌陷区土壤生物学特性及玉米生产性能的影响.利用主成分-数值聚类方法对土壤和玉米的9个指标（脲酶、蔗糖酶、碱性磷酸酶、AM真菌丛枝丰度、总球囊霉素和易提取球囊霉素相关土壤蛋白以及玉米株高、茎粗和地上部干重）进行综合评价,筛选适合该矿区土壤复垦的最优菌种.结果表明,通过主成分分析方法对9个指标进行降维,共提取2个主成分,累计贡献率达81.04%.其中第1主成分以AM真菌丛枝丰度、球囊霉素相关土壤蛋白、脲酶、蔗糖酶及玉米株高、茎粗、地上部干重贡献最大,达到66.68%.第2主成分以磷酸酶活性贡献最大,达到14.36%.以两个主成分得分为新指标进行聚类得到接种不同AM真菌对塌陷矿区土壤培肥效果次序为G.e〉G.i、G.m〉CK.因此接种G.e是改善该采煤塌陷区土壤质量和提高玉米生产性能的最优菌种.
Utilizing a coal mine subsidence soil in Jingshanpo Mining, Jiexiu City, Shanxi Province, the effects of inoculation with arbuscular mycorrhizal（AM） fungi, Glomus etunicatum（G. e）, G. intraradices（G. i）, and G. mosseae（G. m） on reclamation soil biological characteristics and corn production performance were studied. Principal component-cluster analysis was conducted with nine items of soil and maize growth（three types of soil enzymes： urease, alkaline phosphatase, and invertase; abundance of AM fungi; total and easily extractable glomalin-related soil protein; and three parameters of maize growth： plant height, stem diameter, and shoot dry weight） as evaluation indices to comprehensively evaluate the effects of AM fungi inoculation on reclamation of soil fertility in a coal mining subsidence area, so that the most dominant species of AM fungi was screened scientifically and reasonably. The two principal components that reflected 81.04% of the original information quantity were extracted from the initial nine indices. Within the primary group of principal components, AM abundance, total glomalin-related soil protein, easily extractable glomalin-related soil protein, urease activity, invertase activity, plant height, stem diameter, and shoot dry weight, were the major contributors, accounting for a contribution of 66.68%. The secondary principal component, phosphatase activity, accounted for the major contribution of 14.36%. Based on the scores from the two principal components, the hierarchical cluster analysis revealed that the effects of inoculation with AM fungi on coal mining soil quality was in the order of G. e〉 G. i, G. m 〉control check（CK）, i.e., G. e was the most dominantspecies for improving the coal mining subsidence soil quality and benefiting maize growth, whereas G. i and G. m could be an alternative choice when G. e was not available, which had a greater effect than that of CK on reclaimed soil fertility.
作者
郝鲜俊
韩阳
张又丹
高文俊
罗园园
张凯珏
HAO Xianjun;HAN Yang;ZHANG Youdan;GAO Wenjun;LUO Yuanyuan;ZHANG Kaijue(College of Resources and Environment,Shanxi Agricultural University,Taigu 030801,China;National Experimental TeachingDemonstration Centerfor AgriculturalResources andEnvironment,ShanxiAgricultural University,Taigu 030801,China;College of Animal Science and Veterinary Medicine,ShanxiAgricultural University,Taigu 030801,China)
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2018年第4期789-796,共8页
Chinese Journal of Applied and Environmental Biology
基金
国家自然科学基金项目(41601327)
山西省留学回国人员科技活动择优项目
山西省回国留学人员科研项目(2015-061)
山西农业大学科技创新基金(2016ZZ14)资助~~
关键词
AM真菌
采煤塌陷区
土壤质量
作物生长
主成分分析
聚类分析
arbuscular mycorrhizal fungi
coal mining subsidence
soil quality
crop growth
principal component analysis
cluster analysis
作者简介
通讯作者:高文俊,E-mail:wenjungao437@126.com
