摘要
目的观察链脲佐菌素(streptozotocin,STZ)诱导糖尿病大鼠模型肠道菌群结构和丰度的变化。方法雄性SD大鼠25只,随机分为对照组(C)10只、模型组(M)15只。模型组腹腔注射3%的STZ,剂量30 mg/(kg·bw),连续5 d。成模后每月测量体重、血糖等指标。模型成立4周和12周时,分别在同一时间取各组大鼠的新鲜直肠粪便。在Illumina高通量测序平台上对粪便样本中细菌16S r DNA-V3区进行测序,对肠道菌群结构、丰度进行定量分析。结果模型组和对照组获得的优化序列数差异无显著性(P>0.05)。模型组动物肠道菌群的相对丰度指数(Chao1)、多样性指数Shannon与对照组比较降低(P<0.05),Simpson指数与对照组比较升高(P<0.05)。在门水平,变形菌门(Proteobacteria)、蓝藻菌门(Cyanobacteria)、软壁菌门(Tenericutes)、TM7、放线菌门(Actinobacter)相对丰度降低(P<0.05)。在属水平,造模4周,模型组乳杆菌属(Lactobacillus)相对丰度降低(P<0.05);拟杆菌属(Bacteroides)相对丰度升高(P<0.05)。造模12周,模型组乳杆菌属(Lactobacillus)、拟杆菌属(Bacteroides)、瘤胃球菌属(Ruminococcus)相对丰度升高(P<0.05);双歧杆菌属(Bifidobacterium)相对丰度降低(P<0.05)。结论 STZ诱导的SD大鼠糖尿病动物肠道菌群的丰度和多样性降低,为糖尿病与肠道菌群关系研究提供参考。
Objective To investigate the characteristics of gut microbiota in SD rat model of diabetes mellitus induced by streptozotocin（ STZ）. Methods Twenty-five male SD rats were randomly divided into control（ C）（ n = 10）and diabetes（ M）（ n = 15） groups. Rats in the group M received intravenous injection of streptozotocin（ 30 mg/kg） once per day for 5 consecutive days. Fecal samples were collected and examined for the V3 region of the 16 S r DNA gene by Illumina Miseq high-throughput sequencing. The abundance and composition of gut microbiota were analyzed by cluster analysis. Results DNA sequence analysis was successfully performed. The Chao 1 index was lower in the group M than group C（ P〈0. 05）. The Shannon index was lower and the Simpson index was higher in the group M than group C（ P〈0. 05）. At phylum level,the relative abundance of Proteobacteria,Cyanobacteria,Tenericutes,TM7,and Actinobacteriawas lower in the group M than group C（ P〈0. 05）. At genus level,4 weeks after injection,the abundance of Lactobacillus was lower and that of Bacteroidetes was higher in the group M than group C（ P〈0. 05）. 12 weeks after injection,the relative abundance of Lactobacillus,Bacteroides and Ruminococcus was higher and that of Bifidobacterium was lower in the group M than group C（ P〈0. 05）. Conclusions This STZ-induced diabetic SD rat model has a low abundance and diversity of gut microbiota. Quantitative analysis of gut microbiota composition in this animal model provides a basic data for the study of relationship between diabetes mellitus and gut microbiota.
作者
朱华
郭亚茜
杜晓鹏
赵文杰
李彦红
秦川
ZHU Hua, GUO Yaxi, DU Xiaopeng, ZHAO Wenjie, LI Yanhong, QIN Chuan(Key Laboratory of Human Disease Comparative Medicine,Ministry of Health; Institute of Laboratory AnimalScience,Chinese Academy of Medical Sciences （CAMS） ＆ Comparative Medicine Center,Peking Union Medical College（PUMC） Beijing 100021,China)
出处
《中国实验动物学报》
CAS
CSCD
北大核心
2018年第3期349-356,共8页
Acta Laboratorium Animalis Scientia Sinica
基金
医科院重大协同创新项目(No.2017-12M-2-005)~~
关键词
肠道菌群
糖尿病
链脲佐菌素
高通量测序
gut microbiota
diabetes mellitus
streptozotocin
high-throughput sequencing
作者简介
朱华,主任技师,研究方向疾病与肠道微生态.Email：zhuhua0226@vip.sina.com;[通信作者]秦川,教授,博士生导师.研究方向：病理与病理生理学.Email：chuanqin@vip.sina.com
