摘要
目的检测高级别肉瘤的树鼩肠道微生物菌群结构,为探讨肠道微生物菌群结构的改变与肿瘤发生的相关性提供科学依据。方法选取3例患有恶性高级别肉瘤树鼩粪便为CA组,6例健康树鼩粪便为对照NO组。通过16SV3+4区引物进行细菌多样性的鉴定,通过对Reads剪切过滤,OTUs聚类,并进行物种注释及丰度分析,得出两组树鼩肠道微生物结构、丰度以及多样性。结果比较CA组和NO组树鼩肠道微生物结构,多样性指数Chao1指数值、ACE指数值、Shannon指数值差异无统计学意义(P>0.05)。NO组的树鼩肠道菌群OTUs为640种,而CA组为494种,健康树鼩肠道菌群结构呈多样性。在门水平上比较,其中CA组中螺旋体门(Spirochaetes)丰度为0.03%,低于NO组(丰度为0.12%),且差异有统计学意义(P<0.05);CA组厚壁菌门(Firmicutes)丰度为77.79%高于NO组(47.65%),但差异无统计学意义;CA组的拟杆菌门(Bacteroidetes)丰度为0.75%低于NO组(8.31%),但差异无统计学意义。CA组中的乳杆菌属(lactobacillus)是具有统计学意义的生物标识。结论患有高级别肉瘤树鼩的肠道菌群结构丰度和多样性低于健康树鼩,肠道菌群的结构改变及厚壁菌门含量增加可能增加恶性肿瘤的发生。
Objective To investigate the distribution of intestinal microflora in tree shrews with high-grade sarcomas and healthy tree shrews and compare their intestinal microbial structure and diversity,and to explore the relationship of intestinal microflora between high-grade sarcoma and healthy one in tree shrews.Methods Feces from three tree shrews with malignant high-level sarcomas were selected as the CA group,and feces from 6 healthy tree shrews were used as the control group(NO).Bacterial diversity was identified using primers in the 16S V3+4 region.The intestinal microbial structure,abundance and diversity were obtained for both groups by cutting and filtering reads,clustering operational taxonomic units(OTUs),and analyzing the species annotation and abundance.Results The intestinal microbial structure of the tree shrews in the CA and NO groups were compared,and the Chao1 diversity,ACE and Shannon indices did not show significant differences(P>0.05).The NO group contained 640 OTUs,and 494 OTUs were found in the CA group.At the phylum level,the abundance of Spirochaetes in the CA group was 0.03%,which was significantly lower than that in the NO group(0.12%;P<0.05).The abundance of Firmicutes in the CA group was 77.79%,which was higher than that in the NO group(47.65%),but the difference was not statistically significant.The Bacteroidetes abundance in the CA group was 0.75%lower than that in the NO group(8.31%),but this difference was also not statistically significant.Lactobacillus was a statistically significant biomarker in the CA group.Conclusions The structural abundance and diversity of the intestinal microflora in tree shrews with high-grade sarcomas are lower than those in healthy tree shrews.
作者
黄鑫
古文鹏
黎晓慧
李娜
贾杰
李明学
代解杰
HUANG Xin;GU Wenpeng;LI Xiaohui;LI Na;JIA Jie;LI Mingxue;DAI Jiejie(Kunming Medical University,Kunming 650500,China;Chinese Academy of Medical Sciences/Peking UnionMedical College,Institute of Medical Biology,Center of Tree Shrews Germplasm Resource,Yunnan Key Laboratory ofMajor Infections Diseases Vaccine Development,Kunming 650118)
出处
《中国比较医学杂志》
CAS
北大核心
2019年第8期11-16,49,共7页
Chinese Journal of Comparative Medicine
基金
云南省科技人才和平台计划项目(2017HC019)
云南省联合支持国家计划项目(2015GA009)
云南省重大科技专项(2017ZF007)
作者简介
黄鑫(1994—),女,在读硕士,研究方向:人类疾病动物模型的建立及疾病机理研究。E-mail:huangxin_1994@163.com;通信作者:代解杰(1961-),男,研究员,博士生导师。研究方向:人类疾病动物模型的建立及疾病机理研究。E-mail:djj@imbcams.com.cn.
