摘要
目的研究甘草内生菌代谢物的体内外抗炎作用。方法 96孔板中接种巨噬细胞RAW264.7培养,模型组加入1 mg·L^(-1)脂多糖(LPS),各受试药物组分别加入10,20,40 mg·L^(-1)甘草水提液浸膏或内生菌发酵物,2 h后再加入LPS1 mg·L^(-1)培养,检测NO、IL-1β、PGE2的含量。Wistar大鼠90只,随机分为9组,空白组、模型组、阳性对照组给予生理盐水0.01 m L·g^(-1),甘草水煎液组给予甘草水煎液0.95 g·kg^(-1),各内生菌代谢物组给予甘草内生菌发酵物0.95 g·kg^(-1),每日1次,连续灌胃给药5 d。末次给药后1 h,除空白组外,各组大鼠尾静脉注射1 mg·kg^(-1)体质量LPS,制备急性肺炎模型,空白组注射等量生理盐水,阳性对照组按5 mg·kg^(-1)体质量腹腔注射地塞米松给药。6 h后大鼠股动脉取血,检测外周血白细胞计数及血清中TNF-α、IL-6、IL-10的含量。结果与LPS模型组比较,40 mg·L^(-1)时,甘草浸膏组、5组内生菌代谢物组(JTZB006、JTZB018、JTZB059、JTZB060、JTZB063)巨噬细胞RAW264.7分泌的NO、PGE_2、IL-1β的含量显著降低(P<0.05或P<0.01);与同一质量浓度(40 mg·L^(-1))的甘草浸膏组比较,JTZB006、JTZB060组巨噬细胞RAW264.7分泌的PGE2的含量显著降低(P<0.05)。与模型组比较,阳性对照组、甘草水煎液组、3组内生菌代谢物组(JTZB006、JTZB060、JTZB063)大鼠外周血中白细胞数量及血清中TNF-α、IL-6的含量均显著降低,IL-10的含量显著升高(P<0.05或P<0.01);与甘草水煎液组比较,JTZB006、JTZB060、JTZB063组IL-10的含量显著升高(P<0.05或P<0.01)。结论 3株甘草内生菌代谢物(JTZB006、JTZB060、JTZB063)对LPS诱导的炎症模型具有体外及体内抗炎作用。
OBJECTIVE To study anti-inflammatory effect of the metabolites of endophytes isolated from Glycyrrhiza uralensis in vivo and in vitro. METHODS RAW264.7 cells were inoculated onto a 96-well plate. The model group was added with 1 mg·L^-1 LPS for culture, and each test drug group was added with Glycyrrhiza water extracts or the fermentation products of endophytes（10, 20, 40 mg·L^-1） for 2 h of treatment, then LPS（1 mg·L^-1） was added for stimulation. The content of nitric oxide（NO）, Interleukin-1β（IL-1β） and Prostaglandin E2（PGE2） were measured. Ninety Wistar rats were randomly divided into 9 groups, and each group was given the corresponding drug by gavage administration once per day for 5 d. Except the blank group, the other groups were intravenously injected with LPS（1 mg·kg^-1） to establish an acute pneumonia model, while the blank group was injected with the same volume of normal saline. After 6 h, blood was collected from rat femoral artery to detect peripheral white blood cell counts and the contents of serum TNF-α, IL-6 and IL-10. RESULTS Compared with LPS model group, when the mass concentration was maintained at 40 mg·L^-1, the contents of NO, PGE-2 and IL-1β were significantly reduced in extractum Glycyrrhiza group and endophytes fermentation products groups（JTZB006, JTZB018, JTZB059, JTZB060, JTZB063）（P〈0.05 or P〈0.01）. The contents of PGE2 in JTZB006, JTZB060 were significantly lower than those of extractum Glycyrrhiza group at 40 mg·L^-1. Compared with model group, the white blood cell counts and the contents of TNF-α and IL-6 were significantly reduced in positive control group, Glycyrrhiza water decoction group, 3 groups of the fermentation products of endophytes. The contents of IL-10 in JTZB006, JTZB060 and JTZB063 significantly higher than those of Glycyrrhiza water decoction group in rat serum（P〈0.05 or P〈0.01）. CONCLUSION JTZB006, JTZB060 and JTZB063 have anti-inflammatory effects in LPS induced inflammatory model.
作者
杨志军
邓毅
曼琼
杨秀娟
杨延泽
YANG Zhijun;DENG Yi;MAN Qiong;YANG Xiujuan;YANG Yanze(Gansu University of Traditional Chinese Medicine, Lanzhou 730000, Chin)
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2018年第5期633-637,共5页
Chinese Journal of Modern Applied Pharmacy
基金
国家自然科学基金项目(81360633)
关键词
甘草
内生菌代谢物
抗炎
RAW264.7
大鼠
Glycyrrhiza uralensis
the metabolites of endophytes
anti-inflammatory
RAW264.7
rats
作者简介
杨志军，女，博士，副教授Tel：（0931）8765389E-mail：yangzhijun1971@yeah．net
