摘要
从玄参根、主茎、侧枝、叶片中分离得到内生真菌,采用高效液相和液质联用的方法对内生真菌的代谢产物进行分析,筛选能够产哈巴苷或哈巴俄苷的内生真菌,根据菌株的形态学特征,结合分子生物学的系统进化分析进行菌株鉴定。结果表明从玄参中共分离得到内生真菌210株,合并为46个形态型,分属于2纲、9目、13科、17属。HPLC初步筛选出2株内生真菌能够分别产哈巴苷和哈巴俄苷,LC-MS进一步验证得到了能产哈巴俄苷的目标菌株ZJ17,鉴定为Alternaria alternate;以及能产哈巴苷的目标菌株ZJ25,鉴定为A. gaisen。
The endophytic fungi from root,main stem,branch and leaf of Scrophularia ningpoensis were isolated from Zhejiang,whether these strains could yield harpagide or harpagoside were tested by HPLC and LC-MS. According to the morphological characteristic and the similarity of the nucleotide sequence of internal transcribed spacer( ITS) between r DNAs,the strains producing harpagide or harpagoside were identified. The results showed that 210 strains were isolated from the samples,which were classified into 9 orders,13 families and 17 genera by morphological study. Harpagide was detected in endogenous fungi ZJ17 and harpagoside was detected in endogenous fungi ZJ25 by HPLC coupled with LC-MS. ZJ17 was identified as Alternaria alternate and ZJ25 was identified as A.gaisen by its morphology and authenticated by ITS( ITS4 and ITS5 regions and the intervening 5. 8 S rDNA region).
作者
沈湛云
朱波
张泉龙
秦路平
SHEN Zhan-yun;ZHU Bo;ZHANG Quan-long;QIN Lu-ping(College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 311402, China;College of Traditional Chinese Medicine, Zhejiang Pharmaceutical College, Ningbo 315100, China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2019年第10期2046-2050,共5页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(81673528)
浙江省教育厅科研项目(Y201636676)
浙江省教育厅访问工程师校企合作项目(FG2017006)
关键词
玄参
内生真菌
哈巴苷
哈巴俄苷
Scrophularia ningpoensis
endophytic fungi
harpagide
harpagoside
作者简介
沈湛云,博士研究生,研究方向为药用植物与分子生药学,E-mail:274418843@qq.com;通信作者:秦路平,教授,博士生导师,Tel:(0571)61768167,E-mail:qinluping@126.com.
