摘要
该实验运用网络药理学方法研究苦豆子生物碱潜在的药理作用。收集苦豆子主要生物碱成分,然后进行类药性分析、潜在靶点预测和信号通路筛选;借助DAVID分析工具,结合KEGG数据库对通路进行通路注释和分析;使用Cytoscape软件,构建苦豆子生物碱-靶点-通路网络图。结果表明,苦豆子的17种生物碱涉及49个靶点蛋白（共170次）、22条重要信号通路,所建网络药理模型中的3个节点交错连接,代谢通路间相互协调、相互调节。苦豆子生物碱可能对癌症、代谢性疾病、内分泌系统、消化系统、神经系统等方面疾病具有治疗作用。
It was aimed at exploring the potential pharmacological effects of alkaloids in Sophora alopecuroides by means of network pharmacology in this study.The main alkaloids in S.alopecuroides were collected for analysis of drug properties,prediction of potential targets and screening of signaling pathways.DAVID analysis tool combined with KEGG database was used to annotate and analyze the signaling pathway.The alkaloids-targets-signaling pathways network was built through Cytoscape software.Results showed that 17 alkaloids in S.alopecuroides involved 49 targets（170 times in all） and 22 important signaling pathways.Three nodes in model of network pharmacology were cross-linked,and the metabolic pathways were coordinated and regulated by each other.It indicated that alkaloids in S.alopecuroides may have therapeutic effect on diseases of cancer,metabolic disorder,endocrine system,digestive system,nervous system and so on.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2018年第1期160-167,共8页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(81460645,81603227)
关键词
网络药理学
苦豆子
生物碱
靶点
信号通路
network pharmacology
Sophora alopecuroides
alkaloid
target
signaling pathway
作者简介
尚博扬，高级实验师，研究方向为中药药理学，E-mail：313594332@qq．com;[通信作者]王汉卿，副教授，研究方向为中药资源开发与利用，E-mail：wwwhhq@163．com
