摘要
目的:基于IL-6和IL-10探索王清任活血化瘀方剂对重型颅脑损伤大鼠急性期的治疗效果。方法:将120只SPF级雄性SD大鼠随机分成正常组(A)、模型非用药组(B)、血府逐瘀汤组(C)、通窍活血汤组(D)、补阳还五汤组(E),每组24只。采用改良的Feeney's自由落体方法制备重型颅脑损伤大鼠模型,6 h后,C、D、E组分别给予血府逐瘀汤、通窍活血汤、补阳还五汤水提取物灌胃,A、B组给予生理盐水。用药后在第24 h、72 h、168 h等三个时间点分别处死大鼠,采集外周血标本检测血清中IL-6、IL-10的含量。结果:(1)大鼠血清IL-6含量在伤后第24 h明显升高(P<0.05),之后明显下降(P<0.05),至第168 h降至最低值,但仍高于正常组(P<0.05)。血府逐瘀汤组、通窍活血汤组、补阳还五汤组在24 h、72 h两个时间点血清IL-6含量明显低于模型非用药组(P<0.05);在第24 h血府逐瘀汤组与通窍活血汤组的血清IL-6含量明显低于补阳还五汤组(P<0.05)。(2)大鼠血清IL-10含量在伤后三个时间点均明显高于正常组(P<0.05),但三个时间点之间无统计学差异(P>0.05)。血府逐瘀汤组IL-10含量在24 h含量最低,之后逐渐升高,在第168 h含量达到最高值,明显高于模型非用药组(P<0.05);通窍活血汤组和补阳还五汤组IL-10含量与模型非用药组相比无统计学差异(P>0.05)。结论:血府逐瘀汤、通窍活血汤和补阳还五汤可以减轻重型颅脑损伤继发的炎症反应,这一作用可能与活血化瘀药强度呈正相关。
Objective：To explore the treatment effect on IL -6 and IL - 10 at the acute phase after severe tramnatic brain inju- ry in rats by using WANG Qingren Xuefu Zhuyu Decoction. Methods ：A total of 120 male SD SPF rats were randomly divided into normal group （A）, model of non drug group （B）,Xuefu Zhuyu Decoction group （C）, Tongqiao Huoxue Decoction group （D）, Buyang Huanwu Decoction group （E） ,24 rats in each group （n = 24 ）. And thenwe set up the severe traumatic brain injury model in rats by using improved Feency＇ s methods. After 6h, the C, D and E groups were treated with the extract of Xuefu Zhuyu Decoc- tion, Tongqiao Huoxue Decoction and Buyang Huanwu Decoction by gavage ,while A and B groups were given physiological saline. After the administration of drugs in the three time points （24 h,72h, 168h）,we executed the rats and collected the peripheral blood samples to detect the contents of IL - 6 and IL - 10 in serum. Results ： （ 1 ） The levels of IL - 6 in serum were statistically significantly increased after injure in the rats（P 〈0. 05） ,and then declined obviously and down to the lowest level at 168h（P 〈 0. 05 ） , but were still higher than those of normal group（ P 〈 0. 05 ）. The levels of IL - 6 in serum with Tongqiao Huoxue Decoction group,Xuefu Zhuyu Decoction group and Buyang Huanwu Decoction group were lower than that of non drug group in two points （24 h and 72h） （P 〈0. 05）. Serum IL -6 content of Xuefu Zhuyu Decoction group and Tongqiao Huouxe Decoction group were significantly lower than that of Buyang Huanwu Decoction group at 24 h （P 〈 0. 05 ）. （2）The serum IL - 10 levels in the three time points after injury were significantly higher than that in the normal groap（P 〈 O. 05 ）, but there was no statistical difference between the three time points（ P 〉 0. 05 ）. The content of IL - 10 in Xuefu Zhuyu Decoction group was the lowest at 24 h, and then increased gradually, reached the highest value at 168h and it was significantly higher than that of non drug group （P 〈 0. 05 ）. The content of IL - 10 in Tongqiao Huoxue Decoction group and Buyang Huanwu Decoction group had no statistical difference com- pared with that of non drug group（ P 〉 O. 05 ）. Conclusion：Xuefu Zhuyu Decoction, Tongqiao Huoxue Decoction and Buyang Huan- wu Decoction can reduce inflammatory response secondary in severe traumatic brain injury, and this effect may be positive correla- ted with the strength of Xuefu Zhuyu Decoction.
出处
《辽宁中医杂志》
CAS
北大核心
2017年第12期2659-2661,共3页
Liaoning Journal of Traditional Chinese Medicine
基金
国家自然科学基金(NSFC.81460687)
广西壮族自治区卫生厅中医药科技专项(GZLC14-29)
关键词
血府逐瘀汤
通窍活血汤
补阳还五汤
重型颅脑损伤
IL-6
IL-10
Xuefu Zhuyu Decoction
Tongqiao Huoxue Decoction
Buyang Huanwu Decoction
severe traumatic brain injury
IL-6
IL- 10
作者简介
安聪（1990-），男，河南开封人，硕士研究生，研究方向：中西医结合脑病及肺病方向。;通讯作者：潘宇政（1961-），男（壮族），广西南宁人，副教授，硕士，研究方向：中西医结合脑病及肺病方向，E-mail：pyz79298@sina．com。
