摘要
目的探索腹泻型肠易激综合征(IBS-D)大鼠干细胞因子/酪氨酸激酶受体(SCF/C-kit)系统的变化及其与IBS-D免疫功能紊乱的相关性。方法 12只新生大鼠分为正常组和模型组,每组6只。模型组大鼠采用母婴分离、醋酸刺激、束缚应激三因素法制备IBS-D模型。免疫组化法检测两组大鼠结肠组织中SCF和C-kit的蛋白表达;q PCR法检测SCF和C-kit的mRNA表达;统计学分析SCF和C-kit的表达与IBS-D脾系数、胸腺系数、TNF-α、IL-8、IL-10的相关性。结果与正常组相比,模型组的SCF和C-kit的蛋白表达阳性率减少,同时二者的mRNA表达降低。SCF的表达与脾系数、TNF-α、IL-8呈负相关,与IL-10呈正相关;C-kit的表达与胸腺系数、脾系数、TNF-α呈负相关。结论 IBS-D大鼠SCF/C-kit系统异常,可能与IBS-D免疫功能紊乱有关。
Aim To explore the change of stem cell factor-C-kit( SCF-C-kit) system in irritable bowel syndrome with diarrhea( IBS-D) and the correlation between SCF-C-kit system and immune dysfunction.Methods Twelve neonatal rats were divided into normal group and model group with six rats in each group.IBS-D rat model was established through three-factor method( mother-son separation,acetic acid stimulation and constraint). Immunohistochemistry was used to observe in situ protein expressions of SCF and C-kit.q PCR was used for mRNA expressions of SCF and Ckit. Correlation between SCF-C-kit system and spleen coefficients,thymus coefficients,TNF-α,IL-8,IL-10 was analyzed by statistics. Results Compared with normal group,positive rates of SCF and C-kit protein in model group both decreased,and so did mRNA expression. Expression of SCF was negatively correlative with spleen coefficient,TNF-α expression and IL-8 expression,while positively correlative with IL-10 expression. Expression of C-kit was negatively correlated with thymus coefficient, spleen coefficient and TNF-α.Conclusion SCF-C-kit system of IBS-D is abnormal,which may be related with immune dysfunction.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2018年第1期68-72,共5页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目(No 81673842
81703955)
广东省自然科学基金资助项目(No 2014A030313404)
作者简介
柴玉娜（1986-）,女,博士,主管药师,研究方向：分子免疫药理学,通讯作者,E-mail：yuna999@126.com
