摘要
[目的]研究大麻二酚酸合成酶基因(CBDA1)编码的蛋白质序列所包含的生物学信息。[方法]利用生物信息学在线工具及软件分析大麻二酚酸合成酶(CBDAS)的理化性质、亲/疏水性、跨膜结构、信号肽、motif结构域及空间结构等。[结果]CBDAS由544个氨基酸组成,分子量为62 168.42,理论等电点为8.81,是一种稳定的亲水性分泌蛋白,N末端包含1个由28个氨基酸残基组成的信号肽,该蛋白的亚细胞定位在胞外。CBDAS属于氧化还原酶家族,FDA是该酶活性的必需辅因子,CBDAS蛋白中只含有1个低复杂度区域,含有23个磷酸化位点和6个N-糖基化位点,其二级结构主要由α-螺旋、β-转角和无规卷曲组成,三级结构与四氢大麻酚酸合成酶(THCAS)同源性最高。[结论]该研究结果可为今后深入研究CBDAS蛋白的结构特征和功能提供理论参考。
[Objective]To study the biological information contained in the protein sequence encoded by cannabidiolic acid synthase gene（ CBDA1）. [Method]The physicochemical properties,hydrophilicity and hydrophobicity,transmembrane structure domain,the signal peptide,the motif and the spatial structure of cannabidiolic acid synthase（ CBDAS） were analyzed by bioinformatic sever and online tools. [Result]CBDAS consisted of 544 amino acids with molecular weight of 62 168. 42 and a theoretical isoelectric point of 8. 81. It was a stable hydrophilic secretory protein and the N-terminal contained a signal peptide of 28 amino acid residues,moreover,the subcellular localization of CBDAS was extracellular. It was presumed that CBDAS belonged to the family of oxidoreductases and FDA was a necessary cofactor for the activity of CBDAS. In addtion,CBDAS contained one low complexity region,twenty three phosphorylation sites and six N-glycosylation sites. The secondary structure mainly included α-helix,β-turn and random coil. Furthermore,the tertiary structure was the most homologous to tetrahydrocannabinol synthase（ THCAS）. [Conclution]The results could provide a theoretical reference for further study of the structure and functions of CBDAS.
出处
《安徽农业科学》
CAS
2017年第29期144-148,共5页
Journal of Anhui Agricultural Sciences
基金
国家麻类产业体系分子育种项目(CARS-19-E04)
作者简介
常丽（1984-）,女,河南焦作人,助理研究员,博士,从事麻类生物技术研究.
通讯作者：赵立宁,研究员,硕士生导师,从事麻类生物技术研究
通讯作者：李德芳,研究员,博士生导师,从事-年生麻类遗传改良研究.
