摘要
阿魏酸-5-羟化酶(F5H)是木质素生物合成的关键酶之一,它依赖于细胞色素P450催化阿魏酸在5位上发生羟基化反应。采用生物信息学的方法和工具对在GenBank上注册的拟南芥(Arabidopsis thaliana)、油菜(Brassica napus)、杨树(Populus trichocarpa)、番茄(Lycopersicon esculentum)、紫苜蓿(Medicago sativa)、喜树(Camptotheca acuminate)等植物的阿魏酸-5-羟化酶基因的核苷酸序列及推导的氨基酸序列进行分析,包括组成成分、氨基酸翻译后修饰、跨膜拓扑结构域、疏水性/亲水性、蛋白质二级功能结构域等进行分析预测和推断。结果表明,植物F5H是一个具有跨膜结构域的亲水性蛋白,存在于内质网等分泌途径中,α-螺旋和不规则卷曲是其二级结构的主要结构元件,具有细胞色素P450家族特征性结构域及保守功能域。
Ferulate-5-hydroxylase is one of the key enzymes in the biosynthesis of lignins,that can catalyze the cytochrome P450 dependent introduction of a hydroxyl functionality at the 5-position.The nuclei acid sequence and amino acid sequence of ferulate-5-hydroxylase（F5H）from Brassica napus,Arabidopsis thaliana,Populus trichocarpa,Lycopersicon esculentum,Medicago sativa and Camptotheca acuminate,registered in GenBank,were analyzed and predicted by the tools of bioinformatics in the following aspects,including the composition of nuclei acid sequence and amino acid sequence,post-translational modification of amino acids,transmembrane topological structure,hydrophobicity or hydrophilicity,secondary and conversed domain of protein.The results showed that F5H is a hydrophilic and transmembrane protein,which exists in endoplasmic reticulum and other secretory pathway.The main motifs of predicted secondary structure of F5H are alpha helix and random coil.It contains several characteristic and conversed domains of P450 monooxygenases.
出处
《生物技术通报》
CAS
CSCD
北大核心
2010年第6期195-201,211,共8页
Biotechnology Bulletin
作者简介
李嘉，硕士研究生，研究方向：生物化学与分子生物学；E—mail：lijiabio@126．com
通讯作者：孟庆雄，E-mail：qxmeng@sina．com
