摘要
以乙醇为溶剂从蓝莓果皮中提取得到蓝莓皮渣花色苷,采用高效液相色谱分析蓝莓皮渣花色苷组分,以大肠杆菌、金黄色葡萄球菌和枯草芽孢杆菌三种细菌为代表,用牛津杯法考察其抑菌效果,初步确定蓝莓皮渣花色苷的抑菌谱,采用两倍稀释法确定其最低抑菌浓度。采用MTT法检测不同质量浓度花色苷抗结肠癌、胃癌、肝癌细胞增殖活性。蓝莓皮渣花色苷对三种细菌都有抑制作用,其MIC分别为0.63、0.63、1.25 mg/m L。蓝莓皮渣花色苷对三种癌细胞都具有一定的抑制率,其抑制结肠癌、胃癌、肝癌的IC50分别为7.54、13.34、21.05μg/m L。蓝莓皮渣花色苷甘具有一定的抑制细菌和癌细胞增殖的效果,对于开发研制新型抗癌药物具有重要的理论和实际意义。
Anthocyanins were extracted by ethanol from blueberry pomace.The anthocyanin compositions in blueberry pomace were isolated using high performance liquid chromatography （HPLC）.Oxford cup assay was performed to determine the antibacterial effects of anthocyanins using Escherichia coil,Staphylococcus aureus and Bacillus subtilis as the representatives of the three types of bacteria.The antibacterial spectrum of blueberry pomace-derived anthocyanins was preliminarily identified, and the minimum inhibitory concentration was confirmed by sequential 2- fold dilution. Antiproliferation activity of anthocyanins at different concentrations against colorectal cancer, gastric cancer and hepatic carcinoma ceils were determined by MTF assay.Anthocyanins inhibited the three types of bacteria, with MIC values of 0.63,0.63,1.25 mg/mL, for Bacillus subtilis, Staphylococcus aureus and Escherichia coli, respectively.Blueberry pomace-derived anthocyanins also inhibited all three types of cancer cells, with IC50 values of 7.54, 13.34,21.05 μg/mL for colorectal cancer, gastric cancer, and liver cancer cells, respectively.Blueberry pomace derived anthocyanins have certain antibacterial and anticancer activity, and the study has important theoretical and practical significance to reveal mechanism of developing new anticancer drugs.
出处
《食品工业科技》
CAS
CSCD
北大核心
2017年第11期348-351,355,共5页
Science and Technology of Food Industry
基金
浙江省自然科学基金(LY16C200003)
关键词
蓝莓皮渣
花色苷
肿瘤
抑菌活性
blueberry pomace
anthocyanin
tumors
antibacterial effect
作者简介
陈柯斐（1997-），女，苓科生，研究方向：农产品贮藏加工，E—mail：2021521738@qq．com。
通讯作者：王鸿飞（1964-），男，硕士，教授，研究方向：农产品加工与贮藏，E—mail：wailghongfei@nbu．edu．cn。
