摘要
建立了液相色谱-原子荧光法测定大米中无机砷形态的检测方法,对流动相种类、流速、pH值、硼氢化钾浓度及盐酸浓度进行了优化。在8 min内可完成As(Ⅲ)和As(Ⅴ)的分离。当砷酸根和亚砷酸根浓度为0~100ng/ml时,2种形态均可得到良好的线性关系,线性相关系数均大于等于0.999 5,实验室检出限分别为0.006mg/kg和0.009mg/kg,精密度RSD≤6.0%,加标回收率为85.0%~110.7%。该方法减少了样品的分析时间和试剂用量,具有简便、高效和可靠等特点,可用于大米中无机砷形态的检测。
The method was established to determine inorganic arsenic species in rice by liquid chromatography-atomic fluores- cence spectrometry. Types of mobile phase, flow rate, pH, the concentrations of potassium borohydride and hydrochloric acid were optimized. Arsenous acid radical and arsenic acid radical can be separated in 8 min. Arsenous acid radical and arsenic acid radical had good linear relationships with the concentrations of the forms ranging from 0 to 100 ng/ml, and the linear correla- tion coefficients of standard was more than 0. 999 5, the laboratory detection limits of arsenous acid radical and arsenic acid radical were 0. 006 mg/kg and 0. 009 mg/kg, the RSD of the precision of method was less than 6.0%. And the recovery rate of standard addition applied to the sample was 85.0% -110.7 %. Inorganic arsenic species detection by LC-AFS was a simple, efficient and reliable method with less analysis time and reagent dosage, and was feasible for analysis of inorganic arsenic species in rice.
出处
《粮食与饲料工业》
CAS
2017年第2期61-64,共4页
Cereal & Feed Industry
关键词
液相色谱-原子荧光法
大米
无机砷形态
liquid chromatography-atomic fluorescence spectrometry
rice
inorganic arsenic species
作者简介
王婧（1987-），女，硕士，工程师，主要研究粮油食品分析检测。
