摘要
目的筛选新疆维吾尔族、哈萨克族、汉族冠心病相关的差异基因。方法选用病例-对照研究按照1∶1匹配原则,选择经冠状动脉造影检查明确诊断为冠心病患者9例,其中包括维吾尔族3例,哈萨克族3例,汉族3例;对照组选择同期住院经冠状动脉造影检查冠状动脉血管完全正常者9例,两组民族、性别、年龄均匹配;利用人类全基因组表达谱芯片筛查与冠心病相关的差异基因。结果筛查出与冠心病相关的差异基因33条,与汉族冠心病相关的差异基因26条,与维吾尔族冠心病相关的差异基因2条,而哈萨克族未筛选出差异基因表达;经GO和Pathway分析后,差异基因多与免疫反应有关。结论基因芯片筛查的结果表明,对生物学进程有影响的差异基因主要与免疫反应有关。
Aim To sreen differential genes associated with coronary heart disease in Uygur,Kazakh,and Han population of Xinjiang using gene microarray expression profiles.Methods A case-control study including 9 cases（3cases in Uygurs,3 cases in Kazakhs,and 3 cases in Hans） with coronary heart disease confirmed by coronary angiography and 9 gender-,age-,and ethnic-matched normal controls was designed for the present study.Human whole genome expression microarray was used to screen differential genes associated with coronary heart disease.Results 33 genes associated with coronary heart disease were differentially expressed,26 genes associated with coronary heart disease in Han population were differentially expressed,2 genes associated with coronary heart disease in Uygur population were differentially expressed,while the differentially expressed genes were not screened in Kazakh population.Through GO and Pathway analysis,the differentially expressed genes were related to immune response.Conclusion The results of gene microarray screening showed that the differentially expressed genes were mainly related to immune response.
出处
《中国动脉硬化杂志》
CAS
北大核心
2016年第12期1238-1242,共5页
Chinese Journal of Arteriosclerosis
基金
新疆维吾尔自治区自然科学基金医学联合基金资助项目(2015211C166)
关键词
基因芯片
基因表达谱
冠心病
Gene Microarray
Gene Expression Profile
Coronary Heart Disease
作者简介
李霞，博士，副主任医师，研究方向为冠心病的遗传学，E-mail为250456230@qq．com。
王枚，硕士，主治医师，研究方向为老年心血管病诊治。
通讯作者马依彤，博士，主任医师，教授，博士研究生导师，研究方向心血管疾病的介入治疗，E-mail为myt-xj@163．com。
