摘要
多糖为铁皮石斛中的主要有效成分,高效浸出有利于其进一步利用。以水为溶剂,对影响铁皮石斛多糖提取的时间、温度及料液质量比等因素进行了优化研究,并应用苯酚-硫酸法和DNS法考察了铁皮石斛提取液总糖和单糖的提取率,多糖提取率由两者之差计算得出。单因素实验和正交实验结果表明,料液质量比1∶120、浸提温度70℃、浸提时间90min时提取铁皮石斛多糖效果最好,在此工艺条件下,铁皮石斛多糖提取率为55.2%。
Polysaccharide is the most important functional ingredient in Dendrobium officinale Kimura et Migo, and it is important to extract the polysaccharides efficiently for the utilization of D. officinale Kimura et Migo. The extraction conditions such as extraction time, extraction temperature and the ratio of substrate to extraction solution of polysaccharide from D. officinale Kimura et Migo by water using single factor and orthogonal experiments were optimized. The extraction ratio of polysaccharide was calculated by subtract between the total polysaccharide and monosaccharide, which were analyzed by phenol sulfuric acid method and DNS method,respectively. The single factor experiments results showed that the ratio of substrate to water of 1 ： 120, extraction temperature of 70℃ and extraction time of 90 min are the optimal conditions, which are also of the optimal extraction conditions of orthogonal experiments. The identified experiments showed that under the optimal conditions the maximal extraction quantity is of 55.2%.
作者
吴迪
蔡成岗
沙如意
WU Di CAI Chenggang SHA Ruyi(School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, China Zhejiang Provincial Key Laboratory for Chemical and Biological Processing Technology of Farm Produce, Hangzhou 310023, China Zhejiang Collaborative Innovation Center of Chemical and Biological Manufacturing for Agricultural Biological Resources, Hangzhou 310023, China)
出处
《浙江科技学院学报》
CAS
2016年第6期444-449,共6页
Journal of Zhejiang University of Science and Technology
基金
国家级大学生创新创业训练计划项目(201611057014)
浙江科技学院科研启动基金项目(702103E03)
关键词
铁皮石斛
多糖
提取工艺
单因素实验
正交实验
Dendrobium officinale Kimura et Migo
polysaccharide
extraction technology
single factor experiment
orthogonal experiment
作者简介
吴迪（1994-），男，浙江省湖州人，2014级生物工程专业本科生。
通信作者：蔡成岗，副教授，博士，主要从事食品生物技术研究。
