摘要
目的:从蕨菜中分离纯化多糖成分,并进行相对分子质量、单糖组成和免疫调节活性研究。方法:采用热水浸提法提取蕨菜粗多糖,再经脱蛋白、DEAE Sepharose Fast Flow和Sepharose 4B凝胶柱层析得到3种均一多糖组分PLP1、PLP2和PLP3;采用高效液相凝胶渗透色谱法测定均一多糖相对分子质量;采用气相色谱分析均一多糖的单糖组分,采用MTT法和Griess法检测多糖对RAW264.7细胞生长和释放NO能力的影响。结果:PLP1、PLP2和PLP3的相对分子质量分别为6.62×10^4、4.34×10^5、1.88×10^5;PLP1含有鼠李糖、阿拉伯糖、岩藻糖、木糖、甘露糖、葡萄糖和半乳糖,PLP2含有鼠李糖、阿拉伯糖、岩藻糖、木糖、甘露糖和半乳糖,PLP3含有鼠李糖、阿拉伯糖、岩藻糖、木糖、甘露糖和半乳糖。3种多糖均可显著促进RAW264.7细胞的增殖,均具有显著增强RAW264.7细胞释放NO的能力,并呈一定的剂量效应关系。结论:PLP1、PLP2和PLP33个组分均为新的天然来源的均一杂多糖。3种多糖体外均能激活巨噬细胞,呈现显著的免疫调节活性。
Objective:To purify and determine the relative molecular mass,monosacchride compositions and immunomodulating activity of polysaccharides from Pteridium aquilinum(Linn.)Kuhn.Methods:The crude polysaccharide was extracted from P.aquilinum with hot water and then purified by DEAE Sepharose Fast Flow ion-exchange and Sepharose 4B gelfiltration chromatography after removing the proteins.The relative molecular mass was analyzed by high-performance gel permeation chromatography.The monosaccharide compositions were investigated by derivative gas chromatography(GC)and the immunomodulating activities were tested by MTT and Griess method.Results:Three homogenous polysaccharides PLP1,PLP2 and PLP3 were obtained from the crude polysaccharide.Their relative molecular masses were 6.62×104,4.34×105,1.88×105,respectively.Monosaccharide composition analysis by GC revealed that PLP1 was composed of rhamnose,arabinose,fucose,xylose,mannose,glucose,and galactose;PLP2 was composed of rhamnose,arabinose,fucose,xylose,mannose,and galactose;and PLP3 was composed of rhamnose,arabinose,fucose,xylose,mannose,and galactose,respectively.The three polysaccharides promoted the cell proliferation at the concentration of 12.5-100μg·mL-1 and significantly induced the production of NO from RAW264.7 cells in a dose-dependent manner.Conclusion:PLP1,PLP2 and PLP3 are three new natural uniform heteropolysaccharides and all have immunomodulating activity.
作者
宋广磊
王奎武
张虹
居先艳
陈坚波
孙红祥
SONG Guang-lei;WANG Kul-wu;ZHANG Hong;JU Xian-yan;CHEN Jian-bo;SUN Hong-xiang(School of Food Science and Biotechnology,Zhejiang Gongshang University,Hangzhou 310018,China;Hangzhou Brachi Technological Services Co.,Ltd,Hangzhou 310051,China;Jinhua Polytechnic College Jinhua 321007,China;College of Animal Sciences,Zhejiang University,Hangzhou 310058,China)
出处
《药物分析杂志》
CAS
CSCD
北大核心
2016年第12期2107-2112,共6页
Chinese Journal of Pharmaceutical Analysis
关键词
蕨菜
天然多糖
分离纯化
相对分子质量
单糖组分
免疫活性
Pteridium aquilinum
natural polysaccharide
isolation and purification
relative molecular mass
monosaccharide constitution
immunomodulating activity
作者简介
第一作者:宋广磊,Tel:(0571)2800897,E-mail:wkxmpc@zjgsu.edu.cn;通信作者:王奎武,Tel:(0571)2800897,E-mail:wkvmpc@zjgsu.edu.cn
