摘要
本研究使用Illumina Hi Seq平台通过转录组测序对蕨菜可食用期的茎(NJ)和蜷缩叶(JY)以及不可食用期的老茎(LJ)和老叶(LY)样本进行序列分析,获得了26.56 Gb数据,76427个unigene,序列平均长度为1013 bp,其中42177(55.19%)个unigene在Nr、Nt、Swiss-Prot、KEGG、KOG、InterPro和GO数据库获得注释信息。重点分析了蕨菜中与萜类生物合成相关途径unigene133个,主要参与萜类骨架生物合成、单萜化合物生物合成、倍半萜化合物生物合成、二萜和四萜化合物生物合成。其中,NJ与JY比对,差异显著表达基因47个,NJ与LJ比对,差异显著表达基因35个,NJ与LY比对,差异显著表达基因43个。这些基因为进一步深入挖掘蕨菜功能基因、研究萜类化合物等天然活性成分和天然毒素等生物合成与代谢调控分子机制提供依据。
Transcriptome sequencing was performed on the edible stem(NJ)and curled leaves(JY)and the old stem(LJ)and old leaves(LY)samples in the inedible period from Pteridium aquilinum based on Illumina HiSeq platform.A total of 26.56 Gb data were obtained by RNA-Seq to the four samples(NJ,JY,LJ,LY)of Pteridium aquilinum,and assembled into 76427 unigenes with an average length of 1013 bp.42177(55.19%)unigenes were annotated in Nr,Nt,Swiss-Prot,KEGG,KOG,InterPro and GO databases.133 unigenes related to the terpenoids were identified,mainly participated in the biosynthesis pathways of terpenoid backbone,monoterpenes,sesquiterpenes,diterpenes and triterpenes.Among them,there are 47 differentially expressed genes in NJ-JY,35 differentially expressed genes in NJ-LJ,and 43 differentially expressed genes in NJ-LY.It provides the basis for the exploration of functional genes,main active components,natural toxins and other biosynthesis and metabolic regulation molecular mechanisms of bracken.
作者
李珊珊
王丹
张美玲
杨镇才
许练
李波
Li Shanshan;Wang Dan;Zhang Meiling;Zhang Zhencai;Xu Lian;Li Bo(Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas,College of Life Sciences and Agriculture and Forestry,Qiqihar University,Qiqihar,161006)
出处
《分子植物育种》
北大核心
2025年第5期1453-1463,共11页
Molecular Plant Breeding
基金
黑龙江省省属高等学校基本科研业务费科研项目(135509501)资助。
关键词
蕨菜
转录组测序
功能注释
萜类化合物
Pteridium aquilinum
Transcriptome sequencing
Functional annotation
Terpenoids
作者简介
通信作者:李珊珊,lishanshan83@163.com。
