摘要
为了研究铝胁迫下水稻质膜H^+-ATPase对硝态氮吸收影响,以‘滇优35号’(粳稻)为试验材料,采用溶液培养法研究50μmol·L^(-1) Al^(3+)胁迫下添加质膜H^+-ATPase的激活剂壳梭孢素(FC)和抑制剂腺苷-5’-单磷酸(AMP)对质膜H^+-ATPase水解活性和H^+-泵活性,质膜H^+-ATPase和14-3-3蛋白的相互作用对水稻NO_3^--N吸收的影响。结果表明,AMP处理使铝胁迫下水稻质膜H^+-ATPase水解活性、H^+-泵的活性,质膜H^+-ATPase和14-3-3蛋白的相互作用下降,H_2O_2和MDA含量增加,从而导致水稻NO_3^--N吸收降低。FC处理可使铝胁迫下水稻质膜H^+-ATPase水解活性、H^+-泵的活性以及质膜H^+-ATPase和14-3-3蛋白的相互作用增加,H_2O_2和MDA含量下降,从而使水稻NO_3^--N吸收增加。这些研究结果表明铝胁迫通过影响质膜H^+-ATPase与14-3-3蛋白互作来影响质膜H^+-ATPase水解活性和氢泵活性以及H_2O_2和MDA含量,进而影响对硝态氮的吸收。
The aim of the experiment was to study the effects of the plasma membrane（PM） H~＋-ATPase on the nitrate（NO_3^-- N） uptake of rice under aluminum（Al） stress. A hydroponic experiment was conducted with the rice hybrid ‘Dian superior 35＇（Hybrid, Japonica） to study the effects of activator Fusicoccin（FC） and inhibitor Adeosine- 5＇-Monophosphate（AMP） of PM H~＋-ATPase on the activities of PM H~＋-ATPase hydrolysis and H~＋-pump and the interaction of PM H~＋- ATPase and 14-3-3 protein on the uptake of NO_3^--N in root of the rice under 50 μmol·L^（-1） Al stress. The results showed that Al combing with AMP treatment could decrease the activities of PM H~＋-ATPase hydrolytic and H~＋-pump and the interaction of PM H~＋-ATPase and 14-3-3 protein and enhanced the contents of H_2O_2 and malondialdehyde（MDA）, which decreased the NO_3^--N uptake; Al combining with FC treatment could increase the activities of both PM H~＋-ATPase hydrolysis and H~＋-pump, decreased the contents of H_2O_2 and MDA, which promoted the NO_3^--N uptake of rice compared with single Al treatment. These results indicated that Al stress affects the activities of PM H~＋-ATPase hydrolytic and H~＋-pump as well as the contents of H_2O_2 and MDA by influencing the interaction of PM H~＋-ATPase and 14-3-3 protein, thereby affecting the absorption of nitrate.
出处
《植物生理学报》
CAS
CSCD
北大核心
2016年第7期1011-1018,共8页
Plant Physiology Journal
基金
国家自然科学基金(31260297和31560351)
云南省九湖水专项治理项目(KKK0201126044)~~
作者简介
通讯作者（E-mail：likunzhi63@126．com）。
